Identification of a role for the sialomucin CD164 in myogenic differentiation by signal sequence trapping in yeast

Molecular and Cellular Biology
Y N LeeRobert S Krauss

Abstract

Determination and differentiation of skeletal muscle precursors requires cell-cell contact, but the full range of cell surface proteins that mediate this requirement and the mechanisms by which they work are not known. To identify participants in cell contact-mediated regulation of myogenesis, genes that encode secreted proteins specifically upregulated during differentiation of C2C12 myoblasts were identified by the yeast signal sequence trap method (K. A. Jacobs, L. A. Collins-Racie, M. Colbert, M. Duckett, M. Golden-Fleet, K. Kelleher, R. Kriz, E. R. La Vallie, D. Merberg, V. Spaulding, J. Stover, M. J. Williamson, and J. M. McCoy, Gene 198:289-296, 1997), followed by RNA expression analysis. We report here the identification of CD164 as a gene expressed in proliferating C2C12 cells that is upregulated during differentiation. CD164 encodes a widely expressed cell surface sialomucin that has been implicated in regulation of cell proliferation and adhesion during hematopoiesis. Stable overexpression of CD164 in C2C12 and F3 myoblasts enhanced their differentiation, as assessed by both morphological and biochemical criteria. Furthermore, expression of antisense CD164 or soluble extracellular regions of CD164 inhibited myogenic ...Continue Reading

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Citations

Jan 30, 2008·The Journal of Biological Chemistry·Gyu-Un BaeRobert S Krauss
Sep 13, 2008·Molecular Plant-microbe Interactions : MPMI·Jorrit-Jan KrijgerStefan G R Wirsel
Nov 2, 2012·Skeletal Muscle·Alessandro FanzaniEugenio Monti
Mar 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jong-Sun KangRobert S Krauss
Oct 12, 2005·Experimental Cell Research·Marc J ChampignySuleiman A Igdoura
Aug 23, 2006·Journal of Cellular Biochemistry·Guang-Qian ZhouSuzanne M Watt
Feb 27, 2004·Cell Biology International·Dave Van den Plas, Joseph Merregaert

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