Identification of a SAR8.2 gene in the susceptible host response of Nicotiana benthamiana to Colletotrichum orbiculare

Functional Plant Biology : FPB
Xue Chan Shan, Paul H Goodwin

Abstract

A SAR8.2 gene, NbSAR8.2m, was obtained from a PCR-selected cDNA subtraction library constructed from mRNA of Nicotiana benthamiana Domin. infected with Colletotrichum orbiculare (Berk & Mont.) von Arx. It is the first SAR8.2 gene described from N. benthamiana and shows relatively high similarity in both the coding and 3'-UTR to NtSAR8.2m of Nicotiana tabacum L. Expression of NbSAR8.2m occurred in healthy plants but was induced 8-fold following infection by C. orbiculare. Virus-induced gene silencing of NbSAR8.2m reduced its expression and resulted in the development of disease symptoms 24 h earlier than in control plants, indicating that NbSAR8.2m affects the length of the biotrophic phase of infection. Both NtSAR8.2m and NbSAR8.2m are unique among the SAR8.2 genes in that they encode for four cysteines near the C-terminus. The conserved cysteines of SAR8.2 genes may indicate roles in stress responses, defence reactions, metal ion homeostasis or other processes.

References

Nov 1, 1992·Molecular Plant-microbe Interactions : MPMI·D AlexanderJ Ryals
Jun 1, 1983·European Journal of Biochemistry·G von Heijne
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D AlexanderE Ward
Jun 23, 1998·The Plant Cell·M T RuizD C Baulcombe
May 14, 1999·Current Opinion in Plant Biology·D C Baulcombe
Sep 12, 2002·Annual Review of Plant Biology·Christopher Cobbett, Peter Goldsbrough

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Datasets Mentioned

BETA
M97194
AAA34117
AAB49767
AAF18935
AAL16783
U67530
AAB98869
AY644732
U64815
SAR8.2

Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

DRAWGRAM
PROTPARS
CONSENSE
PHYLIP
CLUSTAL
SEQBOOT
BLASTX

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