PMID: 8985340Jan 1, 1997Paper

Identification of a sequence within U5 required for human immunodeficiency virus type 1 to stably maintain a primer binding site complementary to tRNA(Met)

Journal of Virology
S M KangCasey D Morrow

Abstract

Initiation of reverse transcription of human immunodeficiency virus type 1 (HIV-1) occurs by extension from the 3' end of a cellular tRNA complexed to the primer binding site (PBS) located near the 5' end of the viral RNA genome. Although the PBSs for all naturally occurring HIV-1 viruses are complementary to the 3'-terminal 18 nucleotides of tRNA(Lys)3, we identified an HIV-1 virus which contained a PBS complementary to the 3' nucleotides of tRNA(Met); the PBS of this virus was not stable upon extended culture and reverted back to the wild type (S.-M. Kang, J. K. Wakefield, and C. D. Morrow, Virology 222:401-414, 1996). To further characterize the virus with a PBS complementary to tRNA(Met), a DNA fragment encompassing the PBS and U5 region from this proviral genome was substituted for the same region in the infectious HIV-1 proviral clone [named pHXB2(AC-Met)]. Three additional proviral genomes were also created: pHXB2(Met), which is isogenic with pHXB2 except for the PBS complementary to tRNA(Met); pHXB2(Met-AC-Met), which contains the PBS sequence complementary to the 3'-terminal nucleotides and the sequence upstream of this PBS in U5 complementary to the anticodon region of tRNA(Met); and pHXB2(Met-C-Met), which contains t...Continue Reading

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Citations

Jan 15, 2005·AIDS Research and Human Retroviruses·Matthew J RendaStephen Dewhurst
Jan 1, 2010·Viruses·Catherine IselRoland Marquet
Apr 15, 2000·The Journal of Biological Chemistry·J M LanchyR Marquet
Jun 19, 2009·Expert Opinion on Biological Therapy·Virendra N PandeyBinay Chaubey
Aug 15, 2012·Journal of Biomolecular Structure & Dynamics·Mallikarjunachari V N UppuladinneRajendra R Joshi
Jun 15, 2004·The Journal of Biological Chemistry·Valérie GoldschmidtRoland Marquet
Aug 27, 2002·The Journal of Biological Chemistry·Valerie GoldschmidtRoland Marquet
May 16, 2000·The Journal of Biological Chemistry·N Beerens, B Berkhout
Jul 30, 2003·Journal of Virology·Nathan J KellyCasey D Morrow
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·B Chan, K Musier-Forsyth
Dec 31, 2020·International Journal of Molecular Sciences·Thomas GremmingerXiao Heng
Nov 30, 2002·Biochimie·Christine BrunelChantal Ehresmann
Oct 29, 1997·Journal of Virology·J Mak, L Kleiman

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