Identification of a set of proteins (C' and C) encoded by the bicistronic P gene of the Indiana serotype of vesicular stomatitis virus and analysis of their effect on transcription by the viral RNA polymerase

Virology
R PelusoM Lock

Abstract

Previous work (C.F. Spiropoulou and S.T. Nichol, 1993, J. Virol. 67, 3103-3110) has demonstrated the existence in cells infected with the New Jersey serotype of vesicular stomatitis virus (VSV) of two small carboxy-coterminal proteins encoded by the P mRNA. These proteins have been named C' and C. We are interested in studying the function of these proteins in the virus life cycle. Toward this end, we have cloned the ORF encoding the potential C' protein of the Indiana serotype as a histidine-tagged fusion protein, purified the expressed protein from Escherichia coli, and used the fusion protein as an immunogen to raise antiserum in a rabbit. We have used this anti-C' protein serum to demonstrate that both of the predicted C' and C proteins are synthesized in cells infected with the Indiana serotype of VSV. In addition we have localized a portion of these proteins to nucleocapsids isolated from infected cells, suggesting that they may play a role in RNA synthesis. Reconstitution of the viral polymerase activity by expressing the L and P protein subunits with or without the C proteins failed to demonstrate any effect of the presence of these latter proteins on reconstituted transcription using purified nucleocapsids as templates...Continue Reading

Citations

Oct 22, 2010·Journal of Virology·Jason KerkvlietMoses Rodriguez
Mar 19, 2013·Virology Journal·Nikos VasilakisRobert B Tesh
Jul 16, 2009·Archives of Virology·Hong-Lian ChenYu-Lin Jiang
Sep 22, 2011·Virus Research·Peter J WalkerKim R Blasdell
Aug 2, 2014·The Journal of General Virology·Laure BellecLaurent Bigarré
Dec 7, 2007·The Journal of Veterinary Medical Science·Yuki KobayashiTakeo Sakai
Feb 14, 2015·PLoS Pathogens·Peter J WalkerNikos Vasilakis
Jun 15, 2011·Anais Da Academia Brasileira De Ciências·Hilda M A Silva, Valéria Gallo
Feb 10, 2000·Molecular Biology and Evolution·I K JordanM A McClure
Jan 1, 2012·Uirusu·Akira Nishizono, Kentaro Yamada

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