Identification of a silicatein(-related) protease in the giant spicules of the deep-sea hexactinellid Monorhaphis chuni

The Journal of Experimental Biology
Werner E G MüllerHeinz C Schröder

Abstract

Silicateins, members of the cathepsin L family, are enzymes that have been shown to be involved in the biosynthesis/condensation of biosilica in spicules from Demospongiae (phylum Porifera), e.g. Tethya aurantium and Suberites domuncula. The class Hexactinellida also forms spicules from this inorganic material. This class of sponges includes species that form the largest biogenic silica structures on earth. The giant basal spicules from the hexactinellids Monorhaphis chuni and Monorhaphis intermedia can reach lengths of up to 3 m and diameters of 10 mm. The giant spicules as well as the tauactines consist of a biosilica shell that surrounds the axial canal, which harbours the axial filament, in regular concentric, lamellar layers, suggesting an appositional growth of the spicules. The lamellae contain 27 kDa proteins, which undergo post-translational modification (phosphorylation), while total spicule extracts contain additional 70 kDa proteins. The 27 kDa proteins cross-reacted with anti-silicatein antibodies. The extracts of spicules from the hexactinellid Monorhaphis displayed proteolytic activity like the silicateins from the demosponge S. domuncula. Since the proteolytic activity in spicule extracts from both classes of sp...Continue Reading

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Citations

Mar 21, 2009·Journal of Bone and Mineral Metabolism·Ermanno Bonucci
Dec 25, 2010·Marine Biotechnology·Galina N VeremeichikYuri N Kulchin
Jan 1, 2012·Scientifica·Daniel Otzen
Aug 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Katsuhiko ShimizuNobuhiro Mori
May 28, 2008·Cellular Microbiology·Michael J Blackman
May 20, 2015·Journal of Molecular Evolution·Ana RiesgoGonzalo Giribet
Apr 4, 2017·Chemistry, an Asian Journal·Mark B Frampton, Paul M Zelisko
Apr 8, 2010·Chembiochem : a European Journal of Chemical Biology·Werner E G MüllerKlaus Peter Jochum
Nov 15, 2018·Scientific Reports·Natalia V PovarovaKonstantin A Lukyanov

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