Identification of a single peridinin sensing Chl-a excitation in reconstituted PCP by crystallography and spectroscopy.

Proceedings of the National Academy of Sciences of the United States of America
Tim SchulteHarry A Frank

Abstract

The peridinin-chlorophyll a-protein (PCP) of dinoflagellates is unique among the large variety of natural photosynthetic light-harvesting systems. In contrast to other chlorophyll protein complexes, the soluble PCP is located in the thylakoid lumen, and the carotenoid pigments outnumber the chlorophylls. The structure of the PCP complex consists of two symmetric domains, each with a central chlorophyll a (Chl-a) surrounded by four peridinin molecules. The protein provides distinctive surroundings for the pigment molecules, and in PCP, the specific environment around each peridinin results in overlapping spectral line shapes, suggestive of different functions within the protein. One particular Per, Per-614, is hypothesized to show the strongest electronic interaction with the central Chl-a. We have performed an in vitro reconstitution of pigments into recombinant PCP apo-protein (RFPCP) and into a mutated protein with an altered environment near Per-614. Steady-state and transient optical spectroscopic experiments comparing the RFPCP complex with the reconstituted mutant protein identify specific amino acid-induced spectral shifts. The spectroscopic assignments are reinforced by a determination of the structures of both RFPCP an...Continue Reading

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Citations

May 8, 2010·Accounts of Chemical Research·Tomás Polívka, Harry A Frank
Aug 8, 2013·The Journal of Physical Chemistry. a·Samuel D Bockenhauer, W E Moerner
May 10, 2011·Physical Chemistry Chemical Physics : PCCP·Daisuke KosumiHideki Hashimoto
Sep 29, 2011·Physical Chemistry Chemical Physics : PCCP·Daniele BoviLeonardo Guidoni
Aug 7, 2012·Physical Chemistry Chemical Physics : PCCP·Enrico SalvadoriDonatella Carbonera
Aug 5, 2015·FEBS Letters·Jing JiangRobert E Blankenship
Sep 18, 2010·European Journal of Cell Biology·Tim SchulteEckhard Hofmann
Aug 26, 2014·Biochimica Et Biophysica Acta·Jing JiangRobert E Blankenship
Apr 14, 2012·Biochimica Et Biophysica Acta·Jing JiangRobert E Blankenship
Mar 27, 2013·Biophysical Journal·Nicole L WagnerRobert R Birge
Sep 10, 2014·Frontiers in Microbiology·Melissa S Roth
Jul 4, 2016·Biochimica Et Biophysica Acta·Jeffrey R ReimersElmars Krausz
Jan 4, 2017·The Journal of Physical Chemistry Letters·Soumen GhoshWarren F Beck
Jan 13, 2017·Physical Chemistry Chemical Physics : PCCP·Daisuke KosumiHideki Hashimoto
Jun 18, 2014·Nature Chemical Biology·Roberta Croce, Herbert van Amerongen
Jan 31, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Carolin König, Johannes Neugebauer
Oct 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Matthew J Guberman-Pfeffer, José A Gascón
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Nov 11, 2016·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Daniele VarsanoEmanuele Coccia
Aug 10, 2018·Nature Communications·Elena MeneghinElisabetta Collini
Jan 13, 2017·Biochimica Et Biophysica Acta. Bioenergetics·Hui-Yuan S ChenDariusz M Niedzwiedzki
Aug 29, 2013·Nano Letters·Oliviero AndreussiBenedetta Mennucci
Jul 7, 2012·The Journal of Physical Chemistry. B·Milan DurchanTomáš Polívka
Sep 20, 2012·The Journal of Physical Chemistry Letters·D KosumiH Hashimoto
Jan 18, 2018·The Journal of Physical Chemistry Letters·Matthew J Guberman-PfefferJosé A Gascón
Sep 29, 2011·The Journal of Physical Chemistry. B·Marilena Di ValentinDonatella Carbonera
Jan 26, 2018·Organic Letters·Naoto KinashiKazuhiko Sakaguchi

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