PMID: 8611598Jan 12, 1996Paper

Identification of a sodium-bicarbonate symport in human platelets

Biochimica Et Biophysica Acta
O A Gende, H E Cingolani

Abstract

Intracellular pH (pH(i)) was measured in human platelets using fluorescent probes. Basal pH(i) was higher in HC(O3-)- buffered solutions (7(7.33 +/- 0.01) than in nominally HCO3- free, Hepes buffered solutions (7.16 +/- 0.01, P < 0.05). Addition of EIPA caused to fall in Hepes, but did not inhibit the increase of pH(i) when platelets maintained in Hepes were transferred to a CO2/HCO3- buffer. After an intracellular acidosis induced by an NH4Cl prepulse, the initial velocity of recovery (d(pH)/dt(i), in pH units/min) was 3.32 +/- 0.69 in Hepes-buffered solution and 2.85 +/- 0.88 in HCO3- media. Taking into account the differences in buffer capacity, the efflux of acid equivalents after 1.2 min was twice as much in the presence of bicarbonate. The addition of 30 mumol/1 EIPA effectively blocked acid efflux (d(pH)/dt(i) = 0.08 +/ 0.04) in a nominally HCO3- free solution, whereas the recovery was reduced but not abolished (d(pH)/dt(i) = 0.37 +/- 0.10, P < 0.05) in the presence of bicarbonate. The stilbene derivative SITS further inhibited the EIPA-resistant pH(i) recovery. Removal of external Na+ inhibited the HCO(3-)-dependent recovery whereas depletion of internal Cl-, did not suppress it. Depolarization of the membrane had no ef...Continue Reading

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Citations

Jul 15, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·G A SagnellaG A MacGregor
Apr 27, 2005·Thrombosis Research·Oscar A Gende
Apr 17, 2013·Physiological Reviews·Mark D Parker, Walter F Boron

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