PMID: 9642193Jun 27, 1998Paper

Identification of a specific chaperone for SptP, a substrate of the centisome 63 type III secretion system of Salmonella typhimurium

Journal of Bacteriology
Y Fu, J E Galán

Abstract

Salmonella typhimurium uses of a type III protein secretion system encoded at centisome 63 of its chromosome to deliver effector molecule into the host cell. These proteins stimulate host cell responses such as reorganization of the actin cytoskeleton and activation of transcription factors. One of these effector proteins is SptP, a tyrosine phosphatase that causes disruption of the host cell actin cytoskeleton. A characteristic feature of many substrates of type III secretion systems is their association with specific cytoplasmic chaperones which appears to be required for secretion and/or translocation of these proteins into the host cell. We report here the identification of SicP, a 13-kDa acidic polypeptide that is encoded immediately upstream of sptP. A loss-of-function mutation in sicP resulted in drastically reduced levels of SptP but did not affect sptP expression, indicating that SicP exerts its effect posttranscriptionally. Pulse-chase experiments demonstrated that the loss of SicP leads to increased degradation of SptP. In addition, we show that SicP binds to SptP directly and that the binding site is located between residues 15 and 100 of the tyrosine phosphatase. Taken together, these results indicate that SicP act...Continue Reading

References

Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M M ZukowskiJ P Lecocq
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·P WattiauG R Cornelis
Apr 1, 1993·Infection and Immunity·K J MacBeth, C A Lee
Apr 1, 1996·Molecular Microbiology·P WattiauG R Cornelis
Apr 1, 1996·Molecular Microbiology·J E Galán
Jan 1, 1996·Annual Review of Cell and Developmental Biology·J E Galán, J B Bliska
May 1, 1997·Molecular Microbiology·L W ChengO Schneewind
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·W D Hardt, J E Galán
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·W D HardtJ E Galán

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Citations

Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·G R Cornelis
Nov 14, 2000·Journal of Bacteriology·P A BronsteinS I Miller
Oct 9, 2002·Molecular Microbiology·Anne-Laure Page, Claude Parsot
Feb 6, 2004·Molecular Microbiology·Sang Ho Lee, Jorge E Galán
Apr 6, 2005·FEMS Microbiology Reviews·Mark J PallenChristopher M Bailey
Jan 22, 2005·Molecular Microbiology·Liliana C Losada, Steven W Hutcheson
Jun 18, 2011·Cellular Microbiology·Leigh A KnodlerOlivia Steele-Mortimer
Aug 2, 2011·Molecular Microbiology·Julie E Button, Jorge E Galán
Sep 12, 2014·Bioscience, Biotechnology, and Biochemistry·Fumio HayashiKenji Oosawa
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·J E Galan, D Zhou
Sep 17, 2009·Molecular Systems Biology·Daniel M WidmaierChristopher A Voigt
Dec 7, 2016·Journal of Bacteriology·Rebecca JohnsonGad Frankel
Oct 6, 2000·Annual Review of Microbiology·G R Cornelis, F Van Gijsegem
Feb 6, 2004·Journal of Bacteriology·Kristin EhrbarWolf-Dietrich Hardt
Sep 5, 2007·Microbiology·Bae Hoon KimYong Keun Park
Apr 16, 2002·Molecular Microbiology·Anne-Laure PageClaude Parsot
Nov 21, 2007·Nature Reviews. Microbiology·Andrea HaragaSamuel I Miller
Jun 24, 2006·The EMBO Journal·Michel LetzelterGuy R Cornelis
Apr 5, 2013·Clinical Microbiology Reviews·Anna Fàbrega, Jordi Vila
Jan 8, 2015·Protein Science : a Publication of the Protein Society·Andrey M GrishinMiroslaw Cygler
May 15, 2004·Molecular Plant-microbe Interactions : MPMI·Libo ShanXiaoyan Tang
Jul 4, 2007·Expert Reviews in Molecular Medicine·Abigail N Layton, Edouard E Galyov
Nov 3, 2020·Immunity, Inflammation and Disease·Pengtao PanJunlei Yin

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