Identification of a specific domain of Porphyromonas gingivalis Hgp44 responsible for adhesion to Treponema denticola

Pathogens and Disease
Kouki YoshikawaKazuyuki Ishihara

Abstract

Interaction between two periodontal pathogens, Porphyromonas gingivalis and Treponema denticola, contributes to plaque biofilm formation. Porphyromonas gingivalis forms aggregates with T. denticola through its adhesion/hemagglutinin domain (Hgp44). In this study, we investigated the specific domain of P. gingivalis Hgp44 responsible for adhesion to T. denticola using expression vectors harboring P. gingivalis Hgp44 DNA sequences encoding amino acid residues 1-419. Six plasmids harboring fragments in this region were generated by PCR amplification and self-ligation, and recombinant proteins r-Hgp44 (residues 1-419), r-Hgp441 (residues 1-124), r-Hgp442 (1-199), r-Hgp443 (1-316), r-Hgp444 (199-419), r-Hgp445 (124-198) and r-Hgp446 (199-316) were produced, as confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. r-Hgp44, r-Hgp443 and r-Hgp446 showed greater adhesion to T. denticola sonicates than the control, as determined by enzyme-linked immunosorbent assay. r-Hgp446 reduced the coaggregation of P. gingivalis and T. denticola. Scanning electron and confocal laser scanning microscopy analyses revealed that r-Hgp446 reduced dual-species biofilm formation. Our results indicate that residues 199-3...Continue Reading

References

Apr 1, 1992·Oral Microbiology and Immunology·L G SimonsonH E Morton
May 1, 1974·Archives of Oral Biology·K Okuda, I Takazoe
Nov 1, 1993·Journal of Periodontal Research·J Y LeeR J Genco
Mar 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P E KolenbranderC V Hughes
Mar 12, 1998·Journal of Clinical Periodontology·S S SocranskyR L Kent
Jun 1, 1994·Periodontology 2000·A D Haffajee, S S Socransky
Oct 6, 2000·Annual Review of Microbiology·P E Kolenbrander
Nov 6, 2001·Microbiology and Immunology·A KamaguchH Baba
Oct 14, 2003·FEMS Microbiology Letters·Masahito HashimotoTomohiko Ogawa
Dec 5, 2003·Journal of Periodontology·Satoru InagakiKatsuji Okuda
Mar 26, 2004·Nature Reviews. Microbiology·Luanne Hall-StoodleyPaul Stoodley
Aug 9, 2005·FEMS Microbiology Letters·Mitsunori YamadaHoward K Kuramitsu
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neil M O'Brien-SimpsonEric C Reynolds
Aug 16, 2006·FEMS Microbiology Letters·Yoonsuk ParkRichard J Lamont
Jan 11, 2007·Infection and Immunity·Lakshmyya KesavaluJeffrey L Ebersole
Nov 3, 2010·FEMS Immunology and Medical Microbiology·Rieko ItoKatsuji Okuda
Mar 25, 2011·Clinical and Vaccine Immunology : CVI·Kyotaro MuramatsuKazuyuki Ishihara
Dec 1, 2012·Molecular Oral Microbiology·V MeuricM Bonnaure-Mallet
Mar 8, 2014·PLoS Pathogens·Kheng H TanEric C Reynolds
Dec 17, 2014·Trends in Molecular Medicine·Richard J Lamont, George Hajishengallis
Jan 6, 2016·Applied and Environmental Microbiology·Daichi KitaKazuyuki Ishihara

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.