PMID: 9523700Apr 2, 1998Paper

Identification of a specific effector of the small GTP-binding protein Rap2

European Journal of Biochemistry
I Janoueix-LeroseyJ de Gunzburg

Abstract

Rap2 is a small GTP-binding protein that belongs to the Ras superfamily and whose function is still unknown. To elucidate Rap2 function, we searched for potential effectors by screening a mouse brain cDNA library in a yeast two-hybrid system using as a bait a Rap2A protein bearing a mutation of Gly to Val at position 12. This strategy lead to the identification of a protein that interacts specifically with Rap2A complexed with GTP, and requires an intact effector domain of Rap2A for interaction; we designated this protein Rap2-interacting protein 8 (RPIP8). Biochemical data obtained from in vitro studies with purified proteins confirmed the genetic results. Mouse RPIP8 consists of 446 amino acids, bears a coiled-coil domain between residues 265 and 313, and is expressed principally in brain. Its human counterpart, of 400 amino acids, exhibits 93.7% identity in their common region. A search for similar sequences in expressed-sequence-tags databanks revealed the presence in human and rodents of mRNAs encoding the 400-residue and 446-residue forms of RPIP8. Furthermore a doublet of 45-50 kDa, corresponding to the 400-residue and 446-residue forms of the protein, was detected by western blotting of mouse brain extracts and lysates ...Continue Reading

Citations

Feb 13, 2001·Trends in Biochemical Sciences·I CallebautJ P Mornon
Aug 3, 2011·The Journal of Biological Chemistry·Ryan M NottinghamSuzanne R Pfeffer
Jan 15, 2002·DNA and Cell Biology·J F LoringR Somogyi
Jul 27, 2000·Molecular and Cellular Biology·Y OhbaM Matsuda
Apr 4, 2007·The Journal of Clinical Investigation·Liesbeth Van WesenbeeckWim Van Hul
Mar 31, 2005·BMC Cell Biology·Michael BayerAngelika Barnekow
Apr 5, 2014·Neuron·Michael AilionErik M Jorgensen
Jun 30, 2005·International Journal of Cancer. Journal International Du Cancer·Selina RaguzErnesto Yagüe
Oct 13, 2006·Forensic Science International : Synergy·Kazuya IkematsuIchiro Nakasono
Mar 17, 2004·The Journal of Cell Biology·James I S MacDonaldSusan O Meakin
Aug 25, 2006·The Journal of Biological Chemistry·Mutsuko Kukimoto-NiinoShigeyuki Yokoyama
May 29, 2012·The Journal of Biological Chemistry·Ryan M NottinghamSuzanne R Pfeffer
Oct 15, 2019·The Journal of Comparative Neurology·Danye JiangMelanie A Samuel
Dec 23, 1999·The Journal of Biological Chemistry·J de RooijJ L Bos
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Sep 3, 2004·The Journal of Biological Chemistry·Kiyohito TairaKen-ichi Kariya
Aug 18, 2001·The Journal of Biological Chemistry·M MariM Cormont
Aug 28, 2020·Frontiers in Cell and Developmental Biology·Rémy Char, Philippe Pierre
Jan 1, 2003·Journal of Cell Science·Emmanuelle Caron
Jul 3, 2021·Cells·Rajakrishnan Veluthakal, Debbie C Thurmond

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