Identification of a specific interaction between the coronavirus mouse hepatitis virus A59 nucleocapsid protein and packaging signal

Virology
R Molenkamp, W J Spaan

Abstract

The coronavirus mouse hepatitis virus (MHV) is an enveloped positive stranded RNA virus. In infected cells MHV produces a 3' coterminal nested set of subgenomic messenger RNAs. Only the genomic RNA, however, is encapsidated by the nucleocapsid protein and incorporated in infectious MHV virions. It is believed that an RNA packaging signal (Ps), present only in the genomic RNA, is responsible for this selectivity. Earlier studies mapped this signal to a 69-nt stem-loop structure positioned in the 3' end of ORF1b. The selective encapsidation mechanism probably initiates by specific interaction of the packaging signal with the nucleocapsid protein. In this study we demonstrate the in vitro interaction of the MHV-A59 nucleocapsid protein with the packaging signal of MHV using gel retardation and UV cross-linking assays. This interaction was observed not only with the nucleocapsid protein from infected cells but also with that from purified virions and from cells expressing a recombinant nucleocapsid protein. The specificity of the interaction was demonstrated by competition experiments with nonlabeled Ps containing RNAs, tRNA, and total cytoplasmic RNA. The results indicated that no virus specific modification of the N-protein or th...Continue Reading

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Citations

Mar 2, 2013·Journal of Virology·Lili Kuo, Paul S Masters
May 8, 2009·Journal of Virology·Kelley R HurstPaul S Masters
Apr 13, 2007·Journal of Virology·Shih-Cheng ChenRené C L Olsthoorn
Mar 9, 2000·Journal of Virology·R MolenkampE J Snijder
May 14, 2005·Journal of Virology·Barbara SchelleVolker Thiel
May 18, 2006·Annual Review of Microbiology·Anette Schneemann
Oct 17, 2001·The Journal of General Virology·M H VerheijeJ J Meulenberg
Feb 15, 2019·Avian Pathology : Journal of the W.V.P.A·Ana MarandinoRuben Pérez
May 8, 2020·Biomedicines·Kenneth Lundstrom
Aug 25, 2020·SN Comprehensive Clinical Medicine·Anuradha Bharara Singh, Nishant Singh
Dec 12, 2020·Pharmaceuticals·Kenana Al AdemSungmun Lee
Jan 19, 2021·Journal of Oral and Maxillofacial Pathology : JOMFP·Sushma Bommanavar, T Smitha
Dec 9, 2020·Molecular Cell·Christiane IsermanAmy S Gladfelter
Sep 11, 1999·Journal of Virology·I J SalanuevaC Risco

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