Identification of a streptococcal penicillin-binding protein that reacts very slowly with penicillin.

Journal of Bacteriology
R FontanaP Canepari

Abstract

Penicillin-binding protein (PBP) 5 of Streptococcus faecium ATCC 9790 has an unusually low affinity for penicillin (50% binding occurred at a penicillin level of 8 micrograms/ml after 60 min of incubation, and the protein only became labeled after 20 min of incubation with high concentrations of radioactive penicillin). PBPs with similar properties are carried by strains of Streptococcus durans, Streptococcus faecalis, and Streptococcus lactis but not by strains of groups A, B, C, and G streptococci or Streptococcus pneumoniae. The strains carrying the slow-reacting PBP demonstrated a sensitivity to penicillin that was several hundred times lower than that of strains not carrying it. Spontaneous mutants with minimal inhibitory concentrations of penicillin of 20, 40, and 80 micrograms/ml were isolated from S. faecium ATCC 9790. They all showed a dramatic increase in the amount of slow-reacting PBP produced. Mutants with increased penicillin resistance were also isolated from wild-type strains of S. durans, S. faecalis, and S. faecium. All of them carried a greater amount of the slow-reacting PBP than that carried by the parent. Finally, it was found that resistant S. faecium ATCC 9790 mutants grew normally in the presence of pen...Continue Reading

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Citations

Feb 1, 1990·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R FontanaG Satta
May 1, 1995·Molecular Microbiology·J M Frère
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·L Daneo-MooreG D Shockman
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Feb 6, 2008·FEMS Microbiology Reviews·André ZapunThierry Vernet
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Jun 22, 2002·The Journal of Biological Chemistry·Jean-Luc MainardiLaurent Gutmann
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Jul 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·R FontanaG Satta
Jul 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·C RobbiP Canepari
Apr 17, 2001·Antimicrobial Agents and Chemotherapy·L B RiceS D Rudin
Jul 25, 2007·The Journal of Biological Chemistry·Jean-Luc MainardiMichel Arthur
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Seiji OnoTetsuro Matsumoto
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Jan 1, 1989·Antimicrobial Agents and Chemotherapy·E A Tonin, R Fontana
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