PMID: 6407013Jun 1, 1983Paper

Identification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species

Proceedings of the National Academy of Sciences of the United States of America
C J InglesA L Greenleaf

Abstract

Using subclones representing 14 kilobase pairs (kb) of DNA from the Drosophila melanogaster RNA polymerase II (EC 2.7.7.6) X-linked genetic locus, RpII, we have identified four poly(A)+ RNA transcripts in adult flies. The DNA encoding only one of these, a 7-kb transcript, cross-hybridized to mammalian DNA. DNA from alpha-amanitin-resistant (AmaR) Chinese hamster ovary (CHO) and human cells was used to transform the temperature-sensitive (TS) RNA polymerase II Syrian hamster mutant TsAF8. The acquisition of the TS+ AmaR RNA polymerase II phenotype was accompanied by the appearance of donor-DNA-specific restriction fragments that cross-hybridize to the D. melanogaster 7-kb transcript DNA. This D. melanogaster DNA and the related DNA detected in mammalian species must therefore be the structural gene for a RNA polymerase II polypeptide.

References

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Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C J Ingles
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Citations

Sep 1, 1986·Somatic Cell and Molecular Genetics·D PravtchevaF H Ruddle
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·W F Loomis
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C J InglesJ D Friesen
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J L CordenM E Dahmus
Jan 1, 1985·CRC Critical Reviews in Biochemistry·A Sentenac
Jan 1, 1985·Environmental Mutagenesis·S H Clark, A Chovnick
Apr 1, 1987·Journal of Virology·L M RangelP Gariglio
Sep 1, 1993·Microbiological Reviews·J Archambault, J D Friesen

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