Identification of a structural motif crucial for infectivity of hepatitis B viruses.

Proceedings of the National Academy of Sciences of the United States of America
Lars StoecklEberhard Hildt

Abstract

Infectious entry of hepatitis B viruses (HBV) has nonconventional facets. Here we analyzed whether a cell-permeable peptide [translocation motif (TLM)] identified within the surface protein of human HBV is a general feature of all hepadnaviruses and plays a role in the viral life cycle. Surface proteins of all hepadnaviruses contain conserved functional TLMs. Genetic inactivation of the duck HBV TLMs does not interfere with viral morphogenesis; however, these mutants are noninfectious. TLM mutant viruses bind to cells and are taken up into the endosomal compartment, but they cannot escape from endosomes. Processing of surface protein by endosomal proteases induces their exposure on the virus surface. This unmasking of TLMs mediates translocation of viral particles across the endosomal membrane into the cytosol, a prerequisite for productive infection. The ability of unmasked TLMs to translocate processed HBV particles across cellular membranes was shown by confocal immunofluorescence microscopy and by infection of nonpermissive cell lines with HBV processed in vitro with endosomal lysate. Based on these data, we propose an infectious entry mechanism unique for hepadnaviruses that involves virus internalization by receptor-media...Continue Reading

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Citations

Oct 8, 2008·Journal of Biochemistry·Naoya KurataAkihiko Kondo
May 12, 2007·Journal of Virology·Charlotte LepèrePhilippe Gripon
Sep 5, 2008·Journal of Virology·Anneke FunkHüseyin Sirma
Aug 28, 2012·Molecular Neurodegeneration·Karin M DanzerPamela J McLean
Feb 22, 2012·PloS One·Lakshmikanth MariyannaJoachim Hauber
Dec 7, 2013·World Journal of Gastroenterology : WJG·Joachim LupbergerEberhard Hildt
Apr 29, 2014·Gastroenterology·Stephan UrbanFabien Zoulim
Sep 29, 2015·Journal of Gastroenterology and Hepatology·C Nelson HayesKazuaki Chayama
Nov 22, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mitsuo YamadaShun'ichi Kuroda
Dec 23, 2008·Enfermedades infecciosas y microbiología clínica·Julie SheldonVicente Soriano
Aug 12, 2008·Journal of Bioscience and Bioengineering·Takeshi KasuyaShun'ichi Kuroda
Jan 22, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joohee JungShun'ichi Kuroda
Aug 19, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yumi TsutsuiHideki Matsui
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anneke FunkHueseyin Sirma
Feb 10, 2011·Neurobiology of Disease·Lydia Alvarez-ErvitiJ Mark Cooper
Aug 30, 2012·Nucleus·Oren KobilerAriella Oppenheim
Sep 1, 2009·Viruses·Stephanie Schädler, Eberhard Hildt
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Oct 9, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Min LianXiaofeng Zheng
Aug 1, 2015·World Journal of Gastroenterology : WJG·Daniela Ploen, Eberhard Hildt
Sep 6, 2020·Cells·Bingfu Jiang, Eberhard Hildt
Jul 10, 2009·Seminars in Cell & Developmental Biology·Urs F Greber, Daniel Puntener

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