Identification of a subset of group B donors reactive with monoclonal anti-A reagent

American Journal of Clinical Pathology
M L BeckM A Kowalski

Abstract

Two red blood cell (RBC) units labeled group B were returned to the source blood center after they had been retyped as AB by a transfusion service. The discrepancy could be reproduced, but only with the use of the transfusion service's reagent, Ortho Diagnostic's anti-A Bioclone (a licensed, blended, murine monoclonal anti-A reagent). RBCs from 35 of 3,458 random group B donors (1%) reacted with the monoclonal anti-A after immediate centrifugation. Reactivity was associated with high serum levels of B-gene-specified transferase and was caused by the MH04 component, a potent anti-A capable of detecting some examples of Ax RBCs. It is probable that the potency of MH04 permitted detection of low levels of A determinants synthesized by the donors' unusually strong B-gene-specified transferase. Transfer of N-acetylgalactosamine by B-gene-specified transferases, reported in vitro, has not been detected previously in vivo. Use of highly sensitive monoclonal reagents may result in clinically ambiguous blood grouping results.

Citations

Mar 16, 2002·Transfusion·Francis RoubinetAntoine Blancher
Apr 17, 2015·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zhong-Qing YuLi-Hua Hu
Jan 1, 1997·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·M L Beck
Jan 1, 1997·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·M L BeckD L Orr

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