PMID: 9168234May 20, 1997Paper

Identification of a synthetic peptide that mimics an HIV glycoprotein 120 envelope conformational determinant exposed following ligation of glycoprotein 120 by CD4

AIDS Research and Human Retroviruses
J WeinbergB F Haynes

Abstract

CD4 ligation of HIV envelope gp120 results in conformational changes in gp120 that lead to exposure of the gp41 fusogenic domain and fusion with the host cell membrane. One determinant at or near the CD4-binding site exposed on gp120 subsequent to CD4 binding is defined by two human MAbs termed 17b and 48d. These MAbs do not block CD4 binding to gp120; rather, their binding to gp120 is upregulated following CD4 binding. To determine if synthetic peptide mimetopes could be found that reflect conformational determinants on the surface of gp120, synthetic gp120 peptides from 10 divergent HIV isolates were screened for their ability to bind to 17b and 48d in ELISAs. Although MAb 48d binds to HIV IIIB recombinant gp120 protein, in our studies 48d selectively bound only to the HIV Can0A V3 peptide and not to HIV IIIB V3 peptide, whereas MAb 17b bound none of the peptides tested. Monoclonal antibody 48d bound to the HIV Can0A V3 peptide both in solid-phase ELISA and in solution in a competitive ELISA, but could not bind to HIV Can0A V3 peptide bound to human T cells. The HIV Can0A V3 peptide induced anti-HIV antibodies in rhesus monkeys that neutralized the laboratory-adapted HIV MN strain but did not induce antibodies that neutralize...Continue Reading

References

Nov 1, 1992·International Journal of Cell Cloning·Q J Sattentau
Jul 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P L NaraJ Goudsmit
Aug 1, 1991·The Journal of Experimental Medicine·Q J Sattentau, J P Moore
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T J PalkerB F Haynes
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J R RuscheP J Fischinger
Feb 1, 1994·Journal of Virology·F Clavel, P Charneau

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