PMID: 8955408Dec 1, 1996Paper

Identification of a third secondary carrier (DcuC) for anaerobic C4-dicarboxylate transport in Escherichia coli: roles of the three Dcu carriers in uptake and exchange

Journal of Bacteriology
E ZientzGottfried Unden

Abstract

In Escherichia coli, two carriers (DcuA and DcuB) for the transport of C4 dicarboxylates in anaerobic growth were known. Here a novel gene dcuC was identified encoding a secondary carrier (DcuC) for C4 dicarboxylates which is functional in anaerobic growth. The dcuC gene is located at min 14.1 of the E. coli map in the counterclockwise orientation. The dcuC gene combines two open reading frames found in other strains of E. coli K-12. The gene product (DcuC) is responsible for the transport of C4 dicarboxylates in DcuA-DcuB-deficient cells. The triple mutant (dcuA dcuB dcuC) is completely devoid of C4-dicarboxylate transport (exchange and uptake) during anaerobic growth, and the bacteria are no longer capable of growth by fumarate respiration. DcuC, however, is not required for C4-dicarboxylate uptake in aerobic growth. The dcuC gene encodes a putative protein of 461 amino acid residues with properties typical for secondary procaryotic carriers. DcuC shows sequence similarity to the two major anaerobic C4-dicarboxylate carriers DcuA and DcuB. Mutants producing only DcuA, DcuB, or DcuC were prepared. In the mutants, DcuA, DcuB, and DcuC were each able to operate in the exchange and uptake mode.

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Citations

Aug 29, 2001·FEMS Microbiology Reviews·D J Kelly, G H Thomas
Aug 9, 2002·The Journal of Biological Chemistry·Ingo G JanauschGottfried Unden
Aug 28, 2009·Molecular Microbiology·Larissa Tetsch, Kirsten Jung
Jul 5, 2011·Journal of Bacteriology·Martina ValentiniKarine Lapouge
Oct 1, 2009·Acta Microbiologica Et Immunologica Hungarica·A E Abo-Amer, A D Altalhi
Feb 3, 2016·Journal of Bacteriology·Dustin J Van HofwegenScott A Minnich
Jul 5, 2017·Bioscience, Biotechnology, and Biochemistry·Keita FukuiKeietsu Abe
Dec 19, 2006·Journal of Bacteriology·Ok Bin Kim, Gottfried Unden
Sep 27, 2000·Journal of Bacteriology·R UllmannA Kröger
Jan 23, 2002·Biochimica Et Biophysica Acta·I G JanauschG Unden
Dec 1, 2004·EcoSal Plus·Gottfried Unden, Alexandra Kleefeld
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Aug 15, 2006·Metabolic Engineering·M CánovasJ L Iborra

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