Abstract
The VpreB and lambda 5 genes encode proteins that associate non-covalently to form the so-called surrogate light (SL) chain. The SL chain complexes with the immunoglobulin heavy chain to form the pre-B cell receptor, which plays a critical role in B cell development. Expression of the murine SL genes is regulated at the level of transcription initiation. Here, we show that a VpreB1 enhancer is located within the 356 bp immediately upstream of the coding sequence. Interestingly, this region exhibits 96% identity to the upstream region of VpreB2. Deletion mapping located the enhancer to between positions -214 and -47 (+1 is the 5'-most transcription initiation site). The enhancer is tissue and differentiation stage specific, and is composed of several DNA elements that are important for its activity. We also show that a transcription factor, early B cell factor, binds to two such elements, and that at least one of these sites is involved in determining enhancer activity.
References
May 29, 1992·Cell·D KitamuraK Rajewsky
Jan 1, 1992·European Journal of Immunology·T OkabeA Kudo
Jan 1, 1992·European Journal of Immunology·T OkabeA Kudo
Apr 4, 1991·Nature·D KitamuraK Rajewsky
Aug 1, 1990·Molecular and Cellular Biology·E V SchmidtP Leder
Aug 11, 1989·Nucleic Acids Research·E SchreiberW Schaffner
Dec 11, 1986·Nature·N Sakaguchi, F Melchers
Sep 10, 1987·Nature·S Pillai, D Baltimore
Oct 1, 1987·European Journal of Immunology·I L Mårtensson, T Leanderson
Feb 1, 1987·Molecular and Cellular Biology·J R de WetS Subramani
Dec 1, 1981·Cell·F AltD Baltimore
Aug 1, 1994·International Immunology·A RolinkF Melchers
Jul 20, 1995·Nature·H Lin, R Grosschedl
Feb 1, 1995·European Journal of Immunology·T H WinklerH Karasuyama
Nov 1, 1994·Molecular and Cellular Biology·K HahmS T Smale
Jun 1, 1994·International Immunology·I L Mårtensson, F Melchers
Feb 1, 1993·Immunology Today·F MelchersA Rolink
May 1, 1993·Genes & Development·J HagmanR Grosschedl
Feb 1, 1996·Immunity·D LöffertK Rajewsky
Apr 1, 1996·European Journal of Immunology·J L DulI L Mårtensson
Jan 1, 1997·European Journal of Immunology·A Mårtensson, I L Mårtensson
Feb 17, 1997·The Journal of Experimental Medicine·I L MårtenssonT H Winkler
Feb 15, 1997·Genes & Development·S L NuttM Busslinger
Mar 1, 1997·European Journal of Immunology·J TianA Kudo
Jul 1, 1997·Immunity·M SigvardssonR Grosschedl
Citations
Dec 21, 2000·Immunology·I L Mårtensson, R Ceredig
Nov 26, 2002·Molecular and Cellular Biology·David LibergPeter Akerblad
Feb 13, 2004·The Journal of Biological Chemistry·Robert MånssonMikael Sigvardsson
Dec 22, 1998·Molecular and Cellular Biology·P AkerbladM Sigvardsson
Apr 13, 2007·European Journal of Immunology·Robert MånssonMikael Sigvardsson
May 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ramiro Gisler, Mikael Sigvardsson
Mar 4, 2000·Molecular Immunology·R GislerM Sigvardsson
Jul 20, 2005·Trends in Immunology·James Hagman, Kara Lukin