Identification of a tissue- and differentiation stage-specific enhancer of the VpreB1 gene

European Journal of Immunology
C PerssonI L Mårtensson

Abstract

The VpreB and lambda 5 genes encode proteins that associate non-covalently to form the so-called surrogate light (SL) chain. The SL chain complexes with the immunoglobulin heavy chain to form the pre-B cell receptor, which plays a critical role in B cell development. Expression of the murine SL genes is regulated at the level of transcription initiation. Here, we show that a VpreB1 enhancer is located within the 356 bp immediately upstream of the coding sequence. Interestingly, this region exhibits 96% identity to the upstream region of VpreB2. Deletion mapping located the enhancer to between positions -214 and -47 (+1 is the 5'-most transcription initiation site). The enhancer is tissue and differentiation stage specific, and is composed of several DNA elements that are important for its activity. We also show that a transcription factor, early B cell factor, binds to two such elements, and that at least one of these sites is involved in determining enhancer activity.

References

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Citations

Feb 13, 2004·The Journal of Biological Chemistry·Robert MånssonMikael Sigvardsson
Dec 22, 1998·Molecular and Cellular Biology·P AkerbladM Sigvardsson
Apr 13, 2007·European Journal of Immunology·Robert MånssonMikael Sigvardsson
May 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ramiro Gisler, Mikael Sigvardsson
Jul 20, 2005·Trends in Immunology·James Hagman, Kara Lukin

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