PMID: 6967232Mar 1, 1980Paper

Identification of a trans-acting function regulation HLA-DR expression in a DR-negative B cell variant

Somatic Cell Genetics
P Gladstone, D Pious

Abstract

Somatic cell hybridizations were performed between an HLA-DR negative variant of a human B lymphoid cell line (B-LCL) and normal unrelated B-LCLs. The HLA-DR codes for polymorphic determinants on a heterodimeric cell surface lymphocyte differentiation glycoprotein. A variant subline which was selected in a single step from a diploid heterozygous DR-1 DR-3 B-LCL had lost expression of both DR-1 and DR-3 and the heretodimer; it has been described earlier. In a fusion with a DR-2 B-LCL, the hybrids expressed DR-2 and reexpressed the DR-1 and DR-3 alleles. Similar results were seen in a fusion with a different normal B-LCL. Hybrid clones from both fusions were tested with a large number of alloantisera and essentially all informative sera showed reexpression. The results show that (1) the variant did not arise by mutations in the structural genes for DR-1 and DR-3; (2) the normal cells are supplying a missing gene product needed for expression of DR; (3) this gene product is capable of acting in trans. Chromosome counts showed that the apparent recessiveness of the variant in the hybrids was not due to chromosomal segregation.

References

Jul 1, 1976·The Journal of Experimental Medicine·R E HumphreysJ L Strominger
Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·C J BarnstableM J Crumpton
Dec 1, 1978·The Journal of Experimental Medicine·R TosiD Pressman
Oct 1, 1978·The Journal of Experimental Medicine·P P JonesH O McDevitt
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M J GiphartJ L Strominger
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A D Bloom, F T Nakamura
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·K SatoJ W Littlefield
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D PiousG Forrest

❮ Previous
Next ❯

Citations

Jan 1, 1990·Immunologic Research·M BoothbyL H Glimcher
Jan 1, 1990·Immunologic Research·B M PeterlinS Tsang
Nov 15, 1987·International Journal of Cancer. Journal International Du Cancer·F MomburgP Möller
Mar 7, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tara M C HornellElizabeth D Mellins
Jan 19, 2012·International Reviews of Immunology·Giuseppina AlojClaudio Pignata
Jul 1, 1985·Immunological Reviews·A J KormanJ L Strominger
Jan 1, 1990·Immunologic Research·C R Hume, J S Lee
Jan 1, 1985·Immunogenetics·R D SalterP Cresswell
Sep 1, 1987·Molecular and Cellular Biology·K E Sullivan, B M Peterlin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.