Identification of a Trypanosoma cruzi antigen that is shed during the acute phase of Chagas' disease

Molecular and Biochemical Parasitology
J L AffranchinoA C Frasch

Abstract

A Trypanosoma cruzi antigen which is shed into the culture medium by the trypomastigote stage of the parasite and detected in blood of acutely infected mice was cloned and characterized. We designate this antigen shed acute phase antigen (SAPA). Five protein bands with apparent molecular masses ranging from 160 to 200 kDa were detected by immunoblotting of plasma from infected mice and in supernatants of cultured trypomastigotes upon reaction with antibodies against SAPA. A serum obtained from a patient acutely infected with Chagas' disease revealed a similar set of polypeptides in supernatants of cultured trypomastigotes when tested by immunoblotting. SAPA seems thus to be a major shed protein during the acute period of the disease. Twenty-six of 28 sera from human acute cases of Chagas' disease tested reacted with SAPA. Conversely, only 8-10% of sera from chronic cases of the disease contained detectable levels of antibody against SAPA. Sera from rabbits infected with six different parasite strains all contained antibodies against SAPA. Antibodies against SAPA are detectable 15 days after the manifestation of acute Chagas' disease symptoms in humans and 15 days post-infection in sera from mice and rabbits. The nucleotide sequ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1986·The American Journal of Tropical Medicine and Hygiene·V Nussenzweig, R S Nussenzweig
Sep 1, 1987·Molecular and Biochemical Parasitology·C F IbañezA C Frasch
Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·A O LuquettiI Rodrigues
Jul 1, 1988·Molecular and Biochemical Parasitology·C F IbañezA C Frasch
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M T RimoldiK Joiner
Jan 1, 1973·Annual Review of Microbiology·Z Brener
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·N NogueiraZ Cohn
Sep 1, 1984·Molecular and Cellular Biochemistry·N S GonzalezI D Algranati
Jun 1, 1982·Cell·P Borst, G A Cross

❮ Previous
Next ❯

Citations

May 1, 1991·World Journal of Microbiology & Biotechnology·J I MorenoJ Zorzópulos
Jan 1, 1995·Parasitology Research·N M Alcântara-Neves, L C Pontes-de-Carvalho
Aug 1, 1991·Molecular and Biochemical Parasitology·G D PollevickD O Sánchez
Oct 1, 1992·Molecular and Biochemical Parasitology·P WinckerS Goldenberg
Nov 1, 1993·Molecular and Biochemical Parasitology·A BuschiazzoD O Sánchez
Apr 1, 1994·Molecular and Biochemical Parasitology·O E CampetellaA C Frasch
May 1, 1996·Molecular and Biochemical Parasitology·C M EgimaS Schenkman
Apr 1, 1990·Parasitology Today·A C Frasch, M B Reyes
Jan 1, 1991·Parasitology Today·A C FraschU Pettersson
Nov 1, 1992·Parasitology Today·O CampetellaA C Frasch
Jun 1, 1993·Parasitology Today·S Schenkman, D Eichinger
Dec 1, 1991·FEMS Microbiology Immunology·M J LevinR Rangel-Aldao
Feb 1, 1997·Acta Tropica·A GruppiE Vottero-Cima
Oct 17, 2006·Parasitology Today·S Tomlinson, J Raper
Apr 4, 2002·Diagnostic Microbiology and Infectious Disease·Tokiko K MatsumotoEufrosina S Umezawa
Jan 1, 1995·Parasitology·E E JazinU Pettersson
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M B ReyesA C Frasch
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C B PooleL A McReynolds
Feb 25, 1992·Proceedings of the National Academy of Sciences of the United States of America·J M BurnsS G Reed
Feb 1, 1992·The Journal of Experimental Medicine·S SchenkmanV Nussenzweig
May 1, 1995·The Journal of Experimental Medicine·M Chuenkova, M E Pereira
Apr 15, 1993·European Journal of Biochemistry·M A Ferrero-GarcíaA J Parodi
Aug 31, 2001·Clinical and Diagnostic Laboratory Immunology·M NakazawaY M Gomes
Jul 16, 2008·Infection and Immunity·Yasuyuki GotoSteven G Reed

❮ Previous
Next ❯

Related Concepts

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
M B ReyesA C Frasch
Molecular and Biochemical Parasitology
G D PollevickD Sánchez
© 2022 Meta ULC. All rights reserved