Identification of a type I Ca2+/Mg2+-dependent endonuclease induced in maize cells exposed to camptothecin

BMC Plant Biology
Nuria Sánchez-Pons, Carlos M Vicient

Abstract

Camptothecin is a plant alkaloid that specifically binds topoisomerase I, inhibiting its activity and inducing double stranded breaks in DNA and activating the cell responses to DNA damage. Maize cultured cells were incubated in the presence of different concentrations of camptothecin. Camptothecin inhibits cultured cell growth, induces genomic DNA degradation, and induces a 32 kDa Ca2+/Mg2+-dependent nuclease activity. This nuclease, we called CaMNUC32, is inhibited by Zn2+ and by acid pH, it is mainly localized in the nucleus and it cleaves single- and double-stranded DNA, with a higher activity against single-stranded DNA. Two-dimensional electrophoresis combined with mass spectrometry suggests that CaMNUC32 is a member of the type I S1/P1 nuclease family. This type of nucleases are usually Zn2+-dependent but our results support previous indications that S1-type nucleases have a wide variety of enzyme activities, including Ca2+/Mg2+-dependent. We have identified and characterized CaMNUC32, a 32 kDa Ca2+/Mg2+-dependent nuclease of the S1/P1 family induced by the topoisomerase I inhibitor camptothecin in maize cultured cells.

References

Sep 21, 1988·Biochimica Et Biophysica Acta·J CastelláF Climent
Oct 1, 1998·Nucleic Acids Research·C A OleykowskiA T Yeung
Feb 24, 2001·Plant Molecular Biology·M SugiyamaH Fukuda
Jun 8, 2001·Annual Review of Biochemistry·J J Champoux
Feb 15, 2003·FEMS Microbiology Reviews·Neelam A Desai, Vepatu Shankar
Dec 31, 2003·The Journal of Biological Chemistry·Fernando DomínguezFrancisco Javier Cejudo
May 14, 2004·Nucleic Acids Research·Bradley J TillSteven Henikoff
Aug 17, 2005·Nature Reviews. Molecular Cell Biology·Kumiko Samejima, William C Earnshaw
Sep 23, 2006·Nature Reviews. Cancer·Yves Pommier
Apr 10, 2007·Biomolecular Engineering·Joerg Reinders, Albert Sickmann
Jun 5, 2007·BMC Biotechnology·Maxim PimkinAnthony T Yeung
Jul 27, 2007·The Plant Journal : for Cell and Molecular Biology·Karine TriquesAbdelhafid Bendahmane
Jul 18, 2008·The New Phytologist·Theresa J Reape, Paul F McCabe
May 8, 2010·Journal of Plant Physiology·Krzysztof LeśniewiczElzbieta Poreba
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Tomáš PodzimekJiří Santrůček
May 21, 2011·BMC Plant Biology·Nuria Sánchez-PonsCarlos M Vicient
Jun 4, 2011·Cell Death and Differentiation·I Hara-Nishimura, N Hatsugai
Jun 8, 2011·Marine Biotechnology·Larissa A BalabanovaValery A Rasskazov
Jun 28, 2011·Current Opinion in Plant Biology·Guillaume TenaJen Sheen
Apr 18, 2012·Journal of Agricultural and Food Chemistry·Chia-Yun KoJei-Fu Shaw
Jun 15, 2012·Journal of Integrative Plant Biology·Hui-Min ChenXin-Qiang He
Oct 30, 2012·BMC Plant Biology·Krzysztof LeśniewiczPrzemysław Wojtaszek
Apr 27, 2013·Plant & Cell Physiology·Krzysztof LesniewiczElzbieta Poreba

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Citations

Dec 13, 2016·Plant Cell Reports·Andrzej KaźmierczakPrzemysław Bernat
Jan 23, 2019·Frontiers in Plant Science·Flávia P FrancoMarcio C Silva-Filho

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