Identification of a unique mu-class glutathione S-transferase in mouse spermatogenic cells

Molecular Reproduction and Development
K D FulcherE M Eddy

Abstract

The fibrous sheath is a major cytoskeletal structure in the principal piece of the mammalian sperm flagellum. Two peptide sequences obtained from a tryptic digest of mouse fibrous sheath proteins exhibited high homology with mu-class glutathione S-transferases (GSTs). Using a DNA probe amplified from degenerate polymerase chain reaction (PCR) primers predicted from these two peptide sequences, a approximately 1.1 kb cDNA clone for fibrous sheath component 2 (Fsc2) was isolated which had 84% nucleic acid and 89% amino acid sequence identity with a previously reported mu-class human GST gene (hGSTM3; Campbell et al., 1990: J Biol Chem 265:4188-9193). Sequences corresponding to those of the two fibrous sheath peptides were present in the protein encoded by the Fsc2 cDNA. Northern analysis with the full length Fsc2 cDNA detected a approximately 1.1 kb mRNA in 12 of 15 somatic tissues examined, as well as in testis and isolated spermatogenic cells. However, 5'(nt--96 to 12) or 3'(nt 637 to 808) Fsc2 probes, containing mostly noncoding sequences, detected a approximately 1.1 kb mRNA abundant in testis and isolated spermatogenic cells, but absent or present at low levels in somatic tissues. Northern analysis with RNA from testes of mi...Continue Reading

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Citations

Jan 18, 2011·Asian Journal of Andrology·Yan-Feng LiJohn C Herr
Feb 28, 2003·Biology of Reproduction·Paula R BrownEdward M Eddy
Sep 11, 2009·Biology of Reproduction·S Mazaud-GuittotB Le Magueresse-Battistoni
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Mar 28, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Kevin R HayesMathew T Pletcher
Dec 7, 2019·Cellular and Molecular Life Sciences : CMLS·Marc LlavaneraMarc Yeste
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Feb 16, 2006·Reproduction, Fertility, and Development·Regina M Turner
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Dec 1, 2006·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·María PazJesús Del Mazo

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