Identification of a vitamin D response element in the proximal promoter of the chicken carbonic anhydrase II gene.

The Journal of Biological Chemistry
I QuéloP Jurdic

Abstract

The carbonic anhydrase II gene, whose transcription is enhanced by 1, 25-dihydroxyvitamin D3 (1,25-(OH)2D3), encodes an important enzyme in bone-resorbing cells derived from the fusion of monocytic progenitors. We analyzed the 1,25-(OH)2D3-mediated activation of the avian gene by transient transfection assays with promoter/reporter constructs into HD11 chicken macrophages and by DNA mobility shift assays. Deletion and mobility shift analyses indicated that the -62/-29 region confers 1,25-(OH)2D3 responsiveness and forms DNA-protein complexes. The addition of an anti-vitamin D receptor (VDR) antibody inhibited binding to this sequence, whereas anti-retinoid X receptor (RXR) antibody generated a lower mobility complex. Therefore, we concluded that this element binds a VDR.RXR heterodimer, but the addition of extra 1,25-(OH)2D3 had no effect on the formation of this complex. Moreover, the use of nuclear extracts from 1,25-(OH)2D3-treated macrophages led to the formation of an additional high mobility complex also composed of VDR.RXR heterodimer. Mutations provided evidence that the 1, 25-(OH)2D3-mediated activation of the carbonic anhydrase II gene is mediated by VDR.RXR heterodimers bound to a DR3-type vitamin D response element ...Continue Reading

References

Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H M Darwish, H F DeLuca
Apr 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B PralletR Baron
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D E RothW S Sly
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A Lomri, R Baron
Aug 1, 1991·Differentiation; Research in Biological Diversity·M Regnier, M Darmon
Dec 20, 1990·The Journal of Steroid Biochemistry and Molecular Biology·P F ConteR Demicheli
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M B DemayH M Kronenberg
Jul 1, 1989·Proceedings of the Society for Experimental Biology and Medicine·T Suda
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S A KernerJ W Pike
Jan 1, 1988·Skin Pharmacology : the Official Journal of the Skin Pharmacology Society·S PillaiP M Elias
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G D RoodmanG R Mundy
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Z Bar-ShavitA J Kahn
Jan 1, 1993·Leukemia Research·J FeldmanM M Brentani
Mar 3, 1995·The Journal of Biological Chemistry·P N MacDonaldR K DeLisle
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·V CavaillèsM G Parker
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·C ZieroldH F DeLuca
Feb 18, 1993·Nature·C CarlbergW Hunziker
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T L TowersL P Freedman
Jul 1, 1993·Genes & Development·T PerlmannR M Evans
Jan 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M H ZhengJ M Papadimitriou
Feb 1, 1993·The Journal of Nutrition·A W Norman, S Hurwitz
Dec 15, 1995·Cell·D J MangelsdorfR M Evans

❮ Previous
Next ❯

Citations

Aug 24, 2018·Systems Biology in Reproductive Medicine·Jovita Mezquita-Pla
Mar 31, 2005·Endocrine Reviews·Sunil NagpalRadhakrishnan Rathnachalam
Aug 9, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ryan M PelisJ Larry Renfro

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.