Identification of acetylcholinesterase inhibitors in water by combining two-dimensional thin-layer chromatography and high-resolution mass spectrometry

Journal of Chromatography. a
Lena StützRudi Winzenbacher

Abstract

Effect-directed analysis (EDA) is increasingly used in environmental monitoring to detect and identify key toxicants. High-performance thin-layer chromatography (HPTLC) has proven to be a very suitable fractionation technique for this purpose. However, HPTLC is limited in its separation efficiency. Thus, separated fractions could still contain many different components and identification of the effective substances remains difficult. Therefore, in this study a workflow for selective EDA with two-dimensional HPTLC in combination with high-performance liquid chromatography-high-resolution mass spectrometry (HPLC-HRMS) was developed. The aim of the workflow was the stepwise reduction of the sample complexity in order to reduce the number of signals that could be responsible for the measured effects. As a consequence, the identification of effective substances should be facilitated. The acetylcholinesterase inhibition assay (AChE assay) for the detection of potential neurotoxic compounds was applied for biotesting. The transfer of effective zones from the first to the second dimension and also to the mass spectrometric measurement was enabled by extraction. A proof of concept was performed by spiking six acetylcholinesterase inhibi...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.