Identification of Acinetobacter isolates in the A. calcoaceticus-A. baumannii complex by restriction analysis of the 16S-23S rRNA intergenic-spacer sequences.

Journal of Clinical Microbiology
L DolzaniC Monti-Bragadin

Abstract

Members of the genus Acinetobacter are reported to be involved in hospital-acquired infections with an increasing frequency. However, clinical laboratories still lack simple methods that allow complete identification of some pathogenic species, i.e., those corresponding to A. baumannii (DNA group or genospecies 2), unnamed genospecies 3 and 13, and two new genospecies that have recently been described. In fact, a complete discrimination between these species is possible only by DNA-DNA hybridization or ribotyping. Both of these techniques are complex and time-consuming and cannot be performed in most clinical laboratories. As a consequence, isolates belonging to these genospecies are often not differentiated and included, together with the environmental genospecies 1, in the A. calcoaceticus-A. baumannii complex. In this report, a simple and rapid method for the identification of the genospecies belonging to the A. calcoaceticus-A. baumannii complex is proposed. It is based on the combined digestion by the restriction endonuclease AluI and NdeII of the DNA fragments resulting from the amplification of the 16S-23S rRNA intergenic spacer sequences. The analysis of 36 strains characterized by DNA-DNA hybridization in previous stud...Continue Reading

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Citations

Aug 1, 1996·European Journal of Biochemistry·E V VinogradovH Brade
May 2, 2012·Virulence·Aoife HowardRoy D Sleator
Mar 19, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Annalisa MilanLucilla Dolzani
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May 9, 1998·Journal of Clinical Microbiology·R PantophletH Brade
Oct 1, 1996·Journal of Clinical Microbiology·C LagatollaC Monti-Bragadin

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