PMID: 16511792Mar 3, 2006Paper

Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody

Veterinary Clinical Pathology
Elizabeth VilliersVictoria Mallows

Abstract

Flow cytometry may be used to determine immunophenotype or lineage of leukemic cells, but few antibodies are available that are specific for cells of monocytic and granulocytic lineage. The purpose of this study was to evaluate the flow cytometric staining patterns of 3 commercial monoclonal antibodies for monocytes and granulocytes in clinically healthy dogs and in dogs with acute myeloid leukemia (AML). Mouse antihuman macrophage antibody (MAC387), mouse anti-human myeloperoxidase (MPO), and a canine neutrophil-specific antibody (NSA) were evaluated using flow cytometry on blood from 6 clinically healthy control dogs, and on blood (n = 7) and/or bone marrow (n = 2) from 8 dogs with AML. A diagnosis of acute leukemia was confirmed by >30% blasts in bone marrow or >30% blasts in peripheral blood, together with bi- or pancytopenia, circulating CD34-positive blast cells, and clinical signs of disease. Leukemic samples also were evaluated using a wide panel of monoclonal antibodies. MAC387 stained neutrophils and monocytes from control dogs, although the staining profiles for the 2 cell types differed. MPO and NSA resulted in strong positive staining of neutrophils; MPO also stained monocytes weakly. Lymphocytes did not stain with...Continue Reading

Citations

Jun 7, 2008·Veterinary Clinical Pathology·Robin W AllisonWilliam Vernau
Oct 2, 2009·Veterinary Clinical Pathology·Mehrdad AmeriTracey Jackson
Jul 4, 2012·Veterinary Clinical Pathology·Josely F FigueiredoKristen R Friedrichs
Jul 2, 2009·American Journal of Veterinary Research·Barrak M PresslerKathleen I Anderson
Nov 4, 2008·Experimental Hematology·Suzanne G UsherLaura Blackwood
Jun 4, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Sarah C RoodeMatthew Breen
Jan 24, 2015·Veterinary Clinical Pathology·Stefano Comazzi
Apr 27, 2012·Veterinary and Comparative Oncology·T RaposoF L Queiroga
Aug 21, 2016·The Veterinary Clinics of North America. Small Animal Practice·Emily D Rout, Paul R Avery
Oct 11, 2016·The Veterinary Clinics of North America. Small Animal Practice·Nicole I Stacy, John W Harvey
Jul 19, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mathios E MylonakisAlexander F Koutinas
Sep 10, 2010·Veterinary Pathology·F Reggeti, D Bienzle
Jul 17, 2008·The Journal of Veterinary Medical Science·Masaharu HisasueTakatsugu Yamada
Sep 3, 2010·Journal of the American Animal Hospital Association·Stefano ComazziPaola Roccabianca
Nov 27, 2014·Australian Veterinary Journal·A MiglioV Mangili
Jul 2, 2021·Veterinary Pathology·Gregory A KraneChristopher L Mariani

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