Identification of adenine modulating AMPK activation in NIH/3T3 cells by proteomic approach

Journal of Proteomics
Guang-Huar YoungHan-Min Chen

Abstract

AMP-activated protein kinase (AMPK) is a metabolic master switch maintaining the energy homeostasis in cells and thought to modulate cellular response to stresses. Adenine as well as a pharmacological AMPK activator, 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), induced the phosphorylation of AMPK and acetyl-CoA carboxylase in NIH/3T3 cells. Administration of adenine or AICAR increased the expression and translocation of glucose transporter 4, enhanced the cellular glucose uptake, and elevated the intracellular ATP level. To better understand the proteomic changes in response to exogenous adenine treatment, we performed two-dimensional difference gel electrophoresis (2DE-DIGE) and grouped protein spots with similar intensities prior to MS analysis. These process allowed us to exclude these constant expressed proteins, reduce the coverage from abundant signals and increase the identification of middle/lower expressed proteins. Bioinformatics analysis on the proteomic alterations suggested that both of adenine and AICAR could induce up-regulation of a panel of proteins associated with glucose metabolism. We also found that adenine upregulated expression of the glycolytic enzyme, hexokinase 2, indicating a link between ad...Continue Reading

References

Jan 1, 1971·Annual Review of Biochemistry·A W Murray
Nov 1, 1996·Bioscience, Biotechnology, and Biochemistry·K YoshiokaH Abe
Oct 14, 2003·Biochemical Pharmacology·Alfonso PompellaAlessandro F Casini
May 10, 2005·Physiology & Behavior·Gabriele V RonnettYajun Tu
Jul 30, 2005·Journal of Cellular Biochemistry·Józefa Wesierska-GadekGerlinde Zerza-Schnitzhofer
May 17, 2008·The Journal of Biological Chemistry·Xiuyun HouMengwei Zang
Oct 15, 2010·Bioscience, Biotechnology, and Biochemistry·Norio YamamotoHitoshi Ashida
Mar 16, 2012·Kidney International·Guang-Huar Young, Vin-Cent Wu
Oct 30, 2012·Chemistry & Biology·D Grahame HardieSimon A Hawley
Feb 27, 2013·Diabetes & Metabolism Journal·Hayley M O'Neill
Aug 2, 2013·Antioxidants & Redox Signaling·Guang-Huar YoungUNKNOWN NSARF group
Mar 20, 2014·American Journal of Physiology. Endocrinology and Metabolism·Jonas Møller KristensenJørgen F P Wojtaszewski
Jun 13, 2014·American Journal of Physiology. Renal Physiology·Chun-Fu LaiKwan-Dun Wu
Jul 12, 2014·Journal of the American Society of Nephrology : JASN·Chiao-Yin SunChin-Chan Lee
Jul 16, 2014·BioMed Research International·Chin-Chen ChenHan-Min Chen

❮ Previous
Next ❯

Citations

Jul 17, 2019·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Guang-Huar YoungChung Y Hsu
Jul 3, 2018·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Prashanta SilwalSeung-Kiel Park
Oct 16, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Hsin-Wu LaiChun-Che Lin
Sep 20, 2019·The Chinese Journal of Physiology·Yi-Feng Yang, Yao-Jen Liang
Mar 7, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Guang-Huar YoungHan-Min Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.