Identification of adenovirus, influenza virus, parainfluenza virus, and respiratory syncytial virus by two kinds of multiplex polymerase chain reaction (PCR) and a shell vial culture in pediatric patients with viral pneumonia.

Yonsei Medical Journal
Jong-Han LeeHyon-Suk Kim

Abstract

Early identification of causative agents in lower respiratory infection of pediatric patients can reduce morbidity and prevent an overuse of antimicrobials. Two kinds of multiplex polymerase chain reaction (PCR) and a commercial shell vial viral culture were performed to identify causative agents in pediatric patients. Nasopharyngeal aspirates of 220 children diagnosed with viral pneumonia were obtained. Two kinds of multiplex PCR (Seeplex(TM) RV detection kit, and Labopass(TM) RV detection kit), and a shell vial culture by R-Mix were performed. Positive samples from 220 total samples by two multiplex PCRs were 52.7% and 46.4%, respectively. We also cultured 103 samples that showed positive results of the adenovirus, influenza virus, parainfluenza virus, and respiratory syncytial virus (RSV) by two multiplex PCR. The RSV was most frequently detected in 53.0% (Seeplex) and 51.7% (Labopass) of patients. The detection rate of adenovirus (AdV) was 10.3% and 12.1%, influenza virus (IFV) A and B was 12.5% and 3.4%, and parainfluenza virus (PIFV) 1, 2, and 3 were 2.9% and 2.6%. Shell vial cultures showed concordant results with each multiplex PCR by 96.1% and 77.7%, respectively. Sequencing results were 90% consistent with multiplex P...Continue Reading

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Citations

Jan 28, 2014·The Lancet. Respiratory Medicine·Rasa IzadnegahdarShamim A Qazi
Feb 24, 2011·The Pediatric Infectious Disease Journal·Cristina CalvoInmaculada Casas
Dec 3, 2014·Forensic Science International : Synergy·Heleen la GrangeCorena de Beer
Dec 7, 2013·The Clinical Respiratory Journal·Hanah KimHyun Chan Cho
Jul 1, 2014·Current Opinion in Infectious Diseases·Kyle R BrownbackSteven Q Simpson
Aug 8, 2018·Indian Journal of Medical Microbiology·M Anjaneya SwamyJitendra Tiwari

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Methods Mentioned

BETA
PCR
nucleic
nucleic acid amplification

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )

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