Identification of adrenal genes regulated in a potassium-dependent manner

Journal of Molecular Endocrinology
Alexander DierksFelix Beuschlein

Abstract

Potassium and angiotensin II are the main stimulators of aldosterone secretion from the adrenal cortex. As potassium-induced in vivo gene regulation in the adrenal cortex has not been studied in detail, we applied a stepwise screening approach: first, we investigated the effects of chronic potassium substitution in mice. Microarray analysis of adrenal glands revealed a set of genes (set A) that were counter-regulated in a high potassium (HP) and low potassium substitution group, while others (set B) were highly upregulated in the HP intake group. In a second step, time dependency of expression changes of these pre-defined genes was studied following short-term potassium stimulation experiments in vivo. Thirdly, dose dependency of potassium-induced gene regulation was investigated in vitro. Finally, to provide indirect evidence for the potential relevance of the detected changes for autonomous aldosterone secretion, expression analysis of aldosterone-producing adenomas was compared with normal adrenal glands. While most investigated genes were similarly regulated following long- and short-term potassium stimulation in vivo, observed changes were reproducible in NCI h295R adrenocortical cells mostly for the set of genes identifie...Continue Reading

Citations

Apr 10, 2013·European Journal of Endocrinology·Felix Beuschlein
Aug 16, 2011·Molecular and Cellular Endocrinology·Namita G HattangadyWilliam E Rainey
Jan 16, 2014·Endocrinology·Koshiro NishimotoTsugio Seki
Mar 4, 2021·Journal of Hypertension·Yoshimichi TakedaYoshiyu Takeda
May 1, 2021·International Journal of Molecular Sciences·Yoshimichi TakedaYoshiyu Takeda

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