Identification of Aedes aegypti cis-regulatory elements that promote gene expression in olfactory receptor neurons of distantly related dipteran insects

Parasites & Vectors
Keshava MysoreMolly Duman-Scheel

Abstract

Sophisticated tools for manipulation of gene expression in select neurons, including neurons that regulate sexually dimorphic behaviors, are increasingly available for analysis of genetic model organisms. However, we lack comparable genetic tools for analysis of non-model organisms, including Aedes aegypti, a vector mosquito which displays sexually dimorphic behaviors that contribute to pathogen transmission. Formaldehyde-assisted isolation of regulatory elements followed by sequencing (FAIRE-seq) recently facilitated genome-wide discovery of putative A. aegypti cis-regulatory elements (CREs), many of which could be used to manipulate gene expression in mosquito neurons and other tissues. The goal of this investigation was to identify FAIRE DNA elements that promote gene expression in the olfactory system, a tissue of vector importance. Eight A. aegypti CREs that promote gene expression in antennal olfactory receptor neurons (ORNs) were identified in a Drosophila melanogaster transgenic reporter screen. Four CREs identified in the screen were cloned upstream of GAL4 in a transgenic construct that is compatible with transformation of a variety of insect species. These constructs, which contained FAIRE DNA elements associated wit...Continue Reading

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Citations

Sep 9, 2019·Current Opinion in Insect Science·Suzan MansourianOlena Riabinina
Dec 29, 2020·Frontiers in Genetics·Óscar M LezcanoElena Gómez-Díaz
Jan 30, 2021·Hereditas·Ronald J NowlingMolly Duman-Scheel
Nov 10, 2020··Molly Duman-ScheelRonald J. Nowling

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Methods Mentioned

BETA
FAIRE-seq
transgenic
PCR
Flow Cytometry
transgenics
MDS
dissection

Key Resources (RRID) Mentioned

BDSC_9744

Software Mentioned

FIJI ImageJ
Adobe Photoshop CC
Spot Digital Imaging
Zen

Related Concepts