PMID: 8606160Apr 1, 1996Paper

Identification of Agrobacterium tumefaciens genes that direct the complete catabolism of octopine

Journal of Bacteriology
K ChoS C Winans

Abstract

Agrobacterium tumefaciens R10 was mutagenized by using the promoter probe transposon Tn5-gusA7, and a library of approximately 5,000 transcriptional fusions was screened for octopine-inducible patterns of gene expression. Twenty-one mutants carrying strongly inducible gusA fusions, 20 of which showed defects in the catabolism of octopine or its metabolites, were obtained. One group of mutants could not use octopine as a carbon source, while a second group of mutants could not utilize arginine or ornithine and a third group could not utilize octopine, arginine, ornithine, or proline as a carbon source. Utilization of these compounds as nitrogen sources showed similar but not identical patterns. Fifteen fusions were subcloned together with adjacent DNA. Sequence analysis and further genetic analysis indicated that insertions of the first group are localized in the occ region of the Ti plasmid. Insertions of the second group were localized to a gene encoding ornithine cyclodeaminase. This gene is very similar to, but distinct from, a homolog located on the Ti plasmid. This gene is located immediately downstream from a gene encoding an arginase. Genetic experiments indicated that this arginase gene is essential for octopine and arg...Continue Reading

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Citations

Feb 5, 1998·Molecular Plant-microbe Interactions : MPMI·H Kim, S K Farrand
Mar 6, 2002·The Journal of Biological Chemistry·Reiko Akakura, Stephen C Winans
Dec 3, 2014·Frontiers in Plant Science·Thomas G PlattClay Fuqua
Nov 2, 2004·Journal of Bacteriology·Imke SchröderHarold G Monbouquette
Dec 9, 2020·Biotechnology Advances·Jonas De SaegerStephen Depuydt
Oct 11, 2003·Biotechnology Advances·A ZukerA Vainstein
Jul 1, 2021·Phytochemistry·Tatiana Matveeva, Léon Otten
Jun 24, 2021·FEMS Microbiology Reviews·Victor M HernándezMichael F Dunn

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