Identification of AHBA biosynthetic genes related to geldanamycin biosynthesis in Streptomyces hygroscopicus 17997

Current Microbiology
Weiqing HeYiguang Wang

Abstract

To clone and study the geldanamycin biosynthetic gene cluster in Streptomyces hygroscopicus 17997, we designed degenerate primers based on the conserved sequence of the ansamycin 3-amino-5-hydroxybenzoic acid (AHBA) synthase gene. A 755-bp polymerase chain reaction product was obtained from S. hygroscopicus 17997 genomic DNA, which showed high similarity to ansamycin AHBA synthase genes. Through screening the cosmid library of S. hygroscopicus 17997, two loci of separated AHBA biosynthetic gene clusters were discovered. Comparisons of sequence homology and gene organization indicated that the two AHBA biosynthetic gene clusters could be divided into a benzenic and a naphthalenic subgroup. Gene disruption demonstrated that the benzenic AHBA gene cluster is involved in the biosynthesis of geldanamycin. However, the naphthalenic AHBA genes in the genome of Streptomyces hygroscopicus 17997 could not complement the deficiency of the benzenic AHBA genes. This is the first report on the AHBA biosynthetic gene cluster in a geldanamycin-producing strain.

References

Sep 1, 1970·The Journal of Antibiotics·C DeBoerD H Peterson
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y XueD H Sherman
Apr 26, 2000·European Journal of Human Genetics : EJHG·C Pérez-CerdáM Ugarte
Jan 24, 2002·Journal of the American Chemical Society·Jiantao Guo, J W Frost
Apr 3, 2002·Trends in Molecular Medicine·Len Neckers
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tin-Wein YuHeinz G Floss
Sep 5, 2002·Journal of the American Chemical Society·Jiantao Guo, John W Frost
Feb 15, 2003·FEMS Microbiology Letters·Andreas RascherC Richard Hutchinson

❮ Previous
Next ❯

Citations

Sep 24, 2013·Nature Biotechnology·Maulik N ThakerGerard D Wright
Jul 30, 2008·Natural Product Reports·Elizabeth S SattelyChristopher T Walsh
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Ping JiangEckard Wimmer
Dec 24, 2011·Natural Product Reports·Qianjin KangLinquan Bai
Nov 9, 2011·Natural Product Reports·Christopher T WalshBrian D Ames
Feb 7, 2014·Chembiochem : a European Journal of Chemical Biology·Shanren LiYuemao Shen
Dec 6, 2014·Journal of Natural Products·Souvik KusariMichael Spiteller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.