Feb 25, 1976

Identification of albumin as the serum factor essential for the growth of activated human lymphocytes

The Journal of Biological Chemistry
H Spieker-Polet, H Polet

Abstract

Albumin from human, bovine, or rabbit serum supported the growth of concanavalin A-stimulated human thymus-derived lymphocytes equally well. This activity was completely abolished by pepsin digestion. It was shown for bovine serum albumin that the albumin molecule itself, and neither an impurity nor a factor bound to albumin was essential for the growth of lymphocytes. This conclusion was based on observations that the growth-promoting activity could not be removed from albumin, and that the specific activity of albumin remained unaltered after the following procedures: molecular sieving at pH 7.5 at pH 3.0, and in 8 M urea at pH 6.6; ion exchange chromatography at pH 4.3 and in 8 M urea at pH 7.2; isoelectric focusing; charcoal treatment; acetone precipitation; and reduction with 2-mercaptoethanol in the presence of 8 M urea. Dimeric albumin was found to support growth of lymphocytes as well as monomeric albumin, and mercaptalbumin and non-mercaptalbumin were shown to have equal activity.

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Mentioned in this Paper

Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
2-Mercaptoethanol
Neoplasm of Uncertain or Unknown Behavior of Thymus
ALB
Lymphoid Cells
Bos taurus
Disease of Thymus Gland
Acetone Measurement
Pepsin 3
Charcoal

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