Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS

PLoS Neglected Tropical Diseases
Ismail LafriPhilippe Parola

Abstract

Phlebotomine sand flies are known to transmit Leishmania parasites, bacteria and viruses that affect humans and animals in many countries worldwide. Precise sand fly identification is essential to prevent phlebotomine-borne diseases. Over the past two decades, progress in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as an accurate tool for arthropod identification. The objective of the present study was to investigate the usefulness of MALDI-TOF MS as a tool for identifying field-caught phlebotomine. Sand flies were captured in four sites in north Algeria. A subset was morphologically and genetically identified. Six species were found in these areas and a total of 28 stored frozen specimens were used for the creation of the reference spectrum database. The relevance of this original method for sand fly identification was validated by two successive blind tests including the morphological identification of 80 new specimens which were stored at -80°C, and 292 unknown specimens, including engorged specimens, which were preserved under different conditions. Intra-species reproducibility and inter-species specificity of the protein profiles were obtained, allowing us to dist...Continue Reading

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Citations

May 3, 2018·Expert Review of Proteomics·Shyam Sundar, Bhawana Singh
Aug 5, 2017·Parasitology·Amira NebbakPhilippe Parola
Feb 2, 2019·PLoS Neglected Tropical Diseases·Agathe ChavyBenoît de Thoisy
Feb 8, 2018·Parasitology·Fatalmoudou TandinaPhilippe Parola
May 1, 2020·Parasite : Journal De La Société Française De Parasitologie·Basma OuartiPhilippe Parola
Feb 17, 2018·PLoS Neglected Tropical Diseases·Basma El HamzaouiPhilippe Parola
Aug 9, 2020·Parasites & Vectors·Kamila Gaudêncio da Silva SalesFilipe Dantas-Torres
Jul 26, 2017·PLoS Neglected Tropical Diseases·Adama Zan DiarraPhilippe Parola
May 31, 2017·Frontiers in Cellular and Infection Microbiology·Jayaseelan Murugaiyan, Uwe Roesler
Sep 2, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Maureen LarochePhilippe Parola
Dec 22, 2020·Journal of Medical Entomology·Hanene BenyahiaLionel Almeras
Jan 23, 2021·Medical and Veterinary Entomology·L C de Sousa-PaulaF Dantas-Torres
Jan 11, 2021·Malaria Journal·Cécile NabetRenaud Piarroux
Apr 23, 2021·Journal of Medical Entomology·Bruno Leite RodriguesPaloma Helena Fernandes Shimabukuro

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Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

Flex analysis
MALDI
R environment R Development Core Team
Flex
ClinProTools
Flex Control
ChromasPro
Biotyper
- Biotyper
MALDI Biotyper

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