Identification of alternatively spliced Na(+)-Ca2+ exchanger isoforms expressed in the heart

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
I KimC O Lee

Abstract

Alternatively spliced isoforms of Na(+)-Ca2+ exchanger were found from various tissues and species. RT-PCR amplification was performed on the basis of our cloned mouse cardiac Na(+)-Ca2+ exchanger and four alternatively spliced isoforms of Na(+)-Ca2+ exchanger were identified. Three (NCX1.3, NCX1.4, and NCX1.12) of them were first identified in the heart, and one isoform (NCX1.12) was a novel spliced variant. These four spliced variants were present in the embryonic and adult atria and ventricles. Different cell types of the heart expressed different spliced isoforms of Na(+)-Ca2+ exchanger. Southern blot analysis indicated that the Na(+)-Ca2+ exchanger gene existed as a single copy in the mouse genome. Thus, the Na(+)-Ca2+ exchanger isoforms expressed in mouse heart are consistent with being produced by alternative splicing and they may have different functions in various cell types in the mouse heart.

References

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Citations

Dec 13, 2006·Journal of Molecular and Cellular Cardiology·Michael ReppelBernd K Fleischmann
Mar 25, 2004·Journal of Cellular Physiology·I WilliamsP D Bell
Jul 21, 2000·DNA Sequence : the Journal of DNA Sequencing and Mapping·K WakimotoY Nabeshima

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