Identification of amino acid residues important to the neuraminidase activity of the HN glycoprotein of Newcastle disease virus.

Virology
Ronald M IorioM A Bratt

Abstract

Monoclonal antibodies (MAbs) to three overlapping antigenic sites (designated 12, 2, and 23) on the hemagglutinin-neuraminidase glycoprotein (HN) of Newcastle disease virus (NDV) were previously shown to inhibit neuraminidase activity (NA) on neuraminlactose (R. M. Iorio and M. A. Bratt, 1984a, J. Immunol. 133, 2215-2219; R. M. Iorio et al., 1989, Virus Res. 13, 245-262). However, a competitive inhibitor of NA blocks the binding of only MAbs to site 23, suggesting that the domain they recognize may be closely related to the NA site. Antigenic variants selected with site 23 MAbs have single amino acid substitutions at HN residues 192, 193, or 200. Virions of variants, which have a substitution at residue 193 or 200, have alterations in NA which are not attributable to a commensurate change in HN content. A revertant of a temperature-sensitive mutant, which has markedly diminished NA relative to the wild type, has an amino acid substitution at residue 175. A second step revertant having partially restored NA has an additional substitution at residue 192 identical to that in one of the site 23 variants, which, in turn, also makes the revertant resistant to neutralization by site 23 MAbs. Thus, an amino acid substitution at residue...Continue Reading

References

Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R M Iorio, M A Bratt
Sep 1, 1986·The Journal of General Virology·N S MillarP T Emmerson
Jun 1, 1988·Virology·M N WaxhamH M Goodman
Aug 1, 1971·Zeitschrift Für Naturforschung. Teil B. Anorganische Chemie, Organische Chemie, Biochemie, Biophysik, Biologie·P MeindlP Palese
Feb 1, 1973·Journal of Virology·A Scheid, P W Choppin
Oct 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·M A Bratt, W R Gallaher
May 1, 1982·Journal of Virology·M E Peeples, M A Bratt

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Citations

Mar 10, 2001·Trends in Microbiology·T G Morrison
Mar 24, 2016·Journal of Medical Virology·Maja ŠantakDubravko Forčić

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