PMID: 10456868Aug 24, 1999Paper

Identification of amino acid residues of the T-cell epitope of Mycobacterium tuberculosis alpha antigen critical for Vbeta11(+) Th1 cells

Infection and Immunity
Ai KariyoneK Takatsu

Abstract

Stimulation of Mycobacterium tuberculosis-primed lymph node cells from C57BL/6 mice with alpha antigen (also known as antigen 85B and MPT59) induced cell proliferation, production of interleukin 2 and gamma interferon, and expansion of Vbeta11(+) CD4(+) T cells in conjunction with antigen-presenting cells in an I-A(b)-restricted manner. Using a series of 15-amino-acid peptides that overlapped each other by 5 amino acids and spanned the mature alpha antigen, we identified the antigenic epitope for alpha antigen-specific Vbeta11(+) Th1 cells. That peptide (peptide-25), which corresponds to amino acid residues 240 to 254 of alpha antigen, contains a motif that is conserved in I-A(b) and requires processing by antigen-presenting cells. Using peptide-25-reactive Vbeta11(+) T-cell clones and substituted peptide-25 mutants, we determined which amino acid residues within peptide-25 were critical for T-cell receptor (TCR) recognition. Our results showed that the amino acid residues at positions 245, 246, 248, 250, and 251 are important for recognition of TCRVbeta11 and that residues at positions 244, 247, 249, and 252 are I-A(b) contact residues. We also observed that active immunization of C57BL/6 mice with peptide-25 can lead to decre...Continue Reading

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Citations

Mar 4, 2014·Nihon Hansenbyō Gakkai zasshi = Japanese journal of leprosy : official organ of the Japanese Leprosy Association·Toshiki TamuraMasahiko Makino
Oct 7, 2009·Nihon Hansenbyō Gakkai zasshi = Japanese journal of leprosy : official organ of the Japanese Leprosy Association·Toshiki TamuraMasahiko Makino
May 29, 2019·European Journal of Immunology·Shihoko Komine-AizawaMitsuo Honda
Dec 2, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claudia NobregaMargarida Correia-Neves
Dec 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tanner M JohannsSing Sing Way
Feb 18, 2003·Scandinavian Journal of Immunology·S TollefsenH G Wiker
Jan 15, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claudia NobregaMargarida Correia-Neves
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·James W WellsAlistair Noble
Aug 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yoshiro HanyuMieko Kato
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shiki TakamuraYasuhiro Yasutomi
Dec 11, 2007·Tuberculosis·Joel D ErnstAlessandro Sette

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