Identification of amino acids critical for IgE-binding to sequential epitopes of bovine kappa-casein and the similarity of these epitopes to the corresponding human kappa-casein sequence

Allergy
N HanKirsten Beyer

Abstract

The delineation of allergenic (i.e. IgE-binding) epitopes in cow's milk proteins and the amino acids (AAs) critical for IgE-binding is necessary to understand better the structural properties of an allergen and to develop more efficacious immunotherapeutic reagents. Furthermore, this information may enable us to understand better cross-sensitivity between different allergens. Eleven peptides, 10-14 AAs in length, representing the IgE-binding epitopes of kappa-casein were synthesized on a derivatized cellulose membrane with single AA substitutions at each position. Membranes were incubated with pooled sera from 15 milk-allergic patients and individual sera from 10 of the patients included in the pool. For 10/11 allergenic peptides, one to five different single AA substitutions resulted in elimination of IgE-binding of pooled patient sera. Overall at least one mutated peptide could be found for these 10 IgE-binding sites that resulted in a reduction of IgE-binding in at least 80% of the patients who recognized the native protein. Furthermore, the IgE-binding region at AA104-112 on bovine kappa-casein showed a high degree of similarity with the human kappa-casein, respectively, including the AAs critical for IgE-binding. This find...Continue Reading

References

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Citations

Apr 2, 2011·Pediatric Clinics of North America·Jacob D KattanKirsi M Järvinen
Jul 7, 2009·The Journal of Allergy and Clinical Immunology·Jing LinHugh A Sampson
Jul 1, 2008·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·J Vitte, P Bongrand
Nov 19, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K M JärvinenH A Sampson
Nov 3, 2011·Molecular Nutrition & Food Research·Stéphanie MorinStéphane Hazebrouck
Oct 5, 2015·Allergology International : Official Journal of the Japanese Society of Allergology·Hiroaki MatsuoTakanori Taogoshi
Aug 21, 2013·Methods : a Companion to Methods in Enzymology·Heidrun HochwallnerRudolf Valenta
May 5, 2009·Current Opinion in Allergy and Clinical Immunology·Kirsi M Järvinen, Pantipa Chatchatee
Oct 9, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Anna M EhlersHenny G Otten
Jan 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Caterina VillaIsabel Mafra
May 14, 2021·Critical Reviews in Food Science and Nutrition·Lily Jaiswal, Mulumebet Worku

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