PMID: 11921288Mar 29, 2002Paper

Identification of amplified and expressed genes in breast cancer by comparative hybridization onto microarrays of randomly selected cDNA clones

Genes, Chromosomes & Cancer
Jeremy ClarkColin S Cooper

Abstract

Microarray analysis using sets of known human genes provides a powerful platform for identifying candidate oncogenes involved in DNA amplification events but suffers from the disadvantage that information can be gained only on genes that have been preselected for inclusion on the array. To address this issue, we have performed comparative genome hybridization (CGH) and expression analyses on microarrays of clones, randomly selected from a cDNA library, prepared from a cancer containing the DNA amplicon under investigation. Application of this approach to the BT474 breast carcinoma cell line, which contains amplicons at 20q13, 17q11-21, and 17q22-23, identified 50 amplified and expressed genes, including genes from these regions previously proposed as candidate oncogenes. When considered together with data from microarray expression profiles and Northern analyses, we were able to propose five genes as new candidate oncogenes where amplification in breast cancer cell lines was consistently associated with higher levels of RNA expression. These included the HB01 histone acetyl transferase gene at 17q22-23 and the TRAP100 gene, which encodes a thyroid hormone receptor-associated protein coactivator, at 17q11-21. The results demonst...Continue Reading

References

Jul 1, 1992·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·B A GustersonR Reed
Mar 28, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R E GeorgeJ Lunec
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·C CollinsJ W Gray
Aug 12, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Schwab
Feb 6, 1999·International Journal of Cancer. Journal International Du Cancer·R A HarrisM J O'Hare
Aug 7, 1999·The Journal of Biological Chemistry·M Iizuka, B Stillman
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y ZhuJ K Reddy
Mar 4, 2000·Nature Genetics·S A GaytherC Caldas
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Mar 30, 2001·The Journal of Biological Chemistry·T W BurkeJ R Nevins
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·O MonniA Kallioniemi
Nov 17, 2001·Current Opinion in Pharmacology·P Workman

❮ Previous
Next ❯

Citations

Feb 13, 2003·Pathology Oncology Research : POR·Agnes ZvaraLászló G Puskás
Feb 28, 2006·Breast Cancer Research and Treatment·Kyeongmee ParkKyoung-Mee Kim
Oct 17, 2003·Life Sciences·Dao-Hai ZhangEvelyn Siew-Chuan Koay
Apr 2, 2003·Cancer Letters·José M González-SanchoAlberto Muñoz
Aug 19, 2007·Oncogene·S ReaA Akhtar
Oct 24, 2007·Molecular and Cellular Biology·Masayoshi IizukaM Mitchell Smith
Aug 2, 2003·Molecular Pathology : MP·F Al-MullaG Varadharaj
Dec 10, 2002·Annual Review of Physiology·Andrew Y Gracey, Andrew R Cossins
Apr 28, 2012·BMC Genomics·Tara L SpiveyFrancesco M Marincola
Nov 3, 2010·Journal of Experimental & Clinical Cancer Research : CR·Wen-zhong WangJian-xin Gu
Jan 19, 2006·Breast Cancer Research : BCR·Ashleen Shadeo, Wan L Lam
Sep 1, 2004·Pharmacogenomics·Chung-Hae Lee, Pascale F Macgregor
Nov 6, 2004·Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology·Paola ScaruffiGian Paolo Tonini
Sep 17, 2004·The Journal of Pathology·Yann-Jang ChenKuo-Wei Chang
Apr 1, 2006·Alcoholism, Clinical and Experimental Research·James M SikelaMichael F Miles
Apr 10, 2008·The Prostate·Christopher J ShepherdDavid L Hudson
Jun 19, 2007·International Journal of Andrology·N C GoddardJ Shipley
Sep 26, 2006·Biochemical and Biophysical Research Communications·Romuald ContzlerMarcel Huber
Mar 21, 2006·Experimental Cell Research·Mirna Mourtada-MaarabouniGwyn T Williams
Nov 11, 2008·Cancer Genetics and Cytogenetics·Luciane R CavalliPatricia E Berg
Jan 13, 2015·Statistical Methods in Medical Research·Marcelo Azevedo CostaAntônio Pádua Braga
Jan 13, 2004·Oncogene·Andrew FeberColin S Cooper
Nov 28, 2002·Nature Genetics·Rodrigo F ChuaquiMichael R Emmert-Buck
Nov 28, 2002·Nature Genetics·Jonathan R Pollack, Vishwanath R Iyer
Apr 15, 2004·Endocrine Reviews·Cynthia A Heinlein, Chawnshang Chang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.