PMID: 8589031Nov 1, 1995Paper

Identification of amplified DNA sequences in breast cancer and their organization within homogeneously staining regions

Genes, Chromosomes & Cancer
M MulerisB Dutrillaux

Abstract

A modified comparative genomic hybridization (mCGH) technique was used to identify and map amplified DNA sequences in six homogeneously staining regions (hsr) from three primary breast carcinomas. Five different chromosomal regions and bands were identified as sites of amplification: 8p1, 17q21.1, 17q23 (two cases), 19q13.3, and 20q13.3. The mCGH site located on 17q21.1 was demonstrated to correspond to a 50-100-fold amplification of ERBB2. Further in situ hybridization experiments were used to confirm the mCGH results and to characterize the organization of the amplified sequences within the hsr. In five of six instances, two or more chromosomal regions were found amplified in the same hsr. In the tumor with the less modified karyotype, the two hsr comprised DNA sequences from three different chromosomes and showed different patterns of amplification. In the tumor with the most rearranged karyotype, the hsr-carrying chromosomes were formed by the translocation and amplification of sequences from three or four different chromosomal sites. This illustrates the complexity of the amplification process in breast cancers.

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Jan 1, 1994·Cytogenetics and Cell Genetics·M MulerisB Dutrillaux

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Citations

Dec 18, 2001·Genes, Chromosomes & Cancer·Manuel R TeixeiraSverre Heim
Jul 3, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Tian-Bo JinShan-Qu Li
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Mar 5, 2016·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Cuiping ZhangWeiqiang Huang
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Jun 22, 2006·Trends in Genetics : TIG·Donna G Albertson
Sep 18, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Yao WuShu Jiang
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Jan 23, 2021·Genes·Miroslav PribylIva Kubikova
Feb 18, 2021·Cell Chemical Biology·Pauline LejaultDavid Monchaud

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