Identification of an ABC transporter gene that exhibits mRNA level overexpression in fluoroquinolone-resistant Mycobacterium smegmatis

FEBS Letters
S K BanerjeeP K Chakraborti

Abstract

We describe here the PCR amplification of a DNA fragment (mtp1) from Mycobacterium smegmatis using primers derived from consensus sequences of the ABC family of transporters. The fragment encodes amino acid sequences that exhibited significant homology with different ABC transporters. Amino acid sequence alignment of the full length gene with other transporters identified the ABC protein as the B-subunit of the phosphate specific transporter. Strikingly, a M. smegmatis colony which exhibited a high level of ciprofloxacin resistance showed mRNA level overexpression of mtp1. Thus this is the first report in any prokaryote indicating differential expression of an ABC transporter in a fluoroquinolone resistant colony.

References

Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1993·Annual Review of Biochemistry·M M Gottesman, I Pastan
Mar 1, 1993·Nature Genetics·W Gish, D J States
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·H E TakiffW R Jacobs
Sep 13, 1996·Biochemical and Biophysical Research Communications·S K BanerjeeP K Chakraborti
Jan 1, 1996·Annual Review of Biochemistry·J S Blanchard
Oct 1, 1996·Journal of Bacteriology·H Nikaido
Aug 1, 1997·Trends in Microbiology·A A Neyfakh

❮ Previous
Next ❯

Citations

May 28, 2008·Archives of Microbiology·Fatemeh Rafii, Miseon Park
Sep 9, 2000·FEMS Microbiology Reviews·M BraibantJ Content
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Jacek LubelskiArnold J M Driessen
Sep 3, 2013·Journal of Proteomics·Aishwarya Anand RaoSanjeeva Srivastava
Aug 2, 2006·Molecular Microbiology·Jacek LubelskiArnold J M Driessen
Oct 9, 2013·Molecular BioSystems·Minal Patkari, Sarika Mehra
Sep 22, 2001·The Journal of Biological Chemistry·J SarinP K Chakraborti
May 14, 2014·FEMS Microbiology Letters·Jie LuZhuo Zhao
Oct 13, 2021·FEMS Microbiology Reviews·Sille RemmMarkus A Seeger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.