PMID: 9540848Apr 16, 1998Paper

Identification of an active site in the antisecretory factor protein

Biochimica Et Biophysica Acta
Ewa JohanssonIvar Lönnroth

Abstract

The antisecretory factor (AF) is a new regulatory protein, produced in the human pituitary gland, which reverses intestinal fluid secretion induced by cholera toxin. We have previously described the cDNA-cloning and characterization of the expressed gene. The aim of this study was to identify the region responsible for the antisecretory activity in the AF-molecule. The recombinant full-length AF has an increased ability to inhibit hypersecretion after treatment with trypsin, indicating that the activity of AF is achieved by smaller peptide fragments. To localize the active region of AF, we expressed truncated forms of the recombinant protein and examined their antisecretory activity against cholera toxin-induced fluid secretion in rat. Nine recombinant AF peptides and four smaller peptides made by solid phase synthesis were tested. Five of the peptides lacked all activity, whereas seven of them were highly active, a dose between 4 and 15 pmol causing a half-maximal inhibition. All the active peptides contained amino acid 36-42 of the AF sequence, whereas none of the inactive peptides contained this sequence. Our results suggest that the site of the antisecretory activity resides in a small region (I)VCHSKTR between position 35 ...Continue Reading

Citations

Jun 2, 2005·Regulatory Peptides·Malin KimEric Hanse
Nov 14, 2003·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·A LaureniusI Bosaeus
Jun 29, 2000·Annual Review of Biochemistry·D VogesW Baumeister
Nov 26, 2009·Experimental and Molecular Pathology·Darryl Z L'HeureuxGeorge P Tuszynski
Jan 1, 2008·Regulatory Peptides·Ewa JohanssonEva Jennische
Nov 18, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ivar LönnrothJavier Torres
Aug 16, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Eva JennischeIngela Jonson
Jul 28, 2015·Mycoses·C Stecksén-BlicksC E West
Dec 27, 2008·Journal of Immunological Methods·Ewa JohanssonEva Jennische
Sep 7, 2016·Scientific Reports·Masoumeh Dowlatshahi PourPer Malmberg
Aug 6, 2010·Nutrition Research Reviews·Caterina UlgheriFilippo Rossi
Feb 10, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Todd S Davidson, William F Hickey
Oct 13, 2019·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Alfonso ScarpaEttore Cassandro
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Oct 7, 2006·Current Opinion in Gastroenterology·J L Turvill, M J Farthing
Jan 9, 2018·Journal of Molecular Neuroscience : MN·V BazzurroM Robello
Aug 11, 2018·Journal of Neurovirology·Ewa JohanssonMarie Studahl
Mar 8, 2011·Acta Oncologica·Mohamed Al-OlamaEva Jennische
Nov 9, 2019·Upsala Journal of Medical Sciences·Annelie Barrueta TenhunenGaetano Perchiazzi
Feb 13, 2018·Molecular Cancer Research : MCR·Shirin IlkhanizadehAnders I Persson
Oct 8, 2014·Biochemistry·Maria Matson DzeboSandra Rocha

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