PMID: 8608160Mar 29, 1996Paper

Identification of an alkaline sphingomyelinase activity in human bile

Biochimica Et Biophysica Acta
L NybergA Nilsson

Abstract

The hydrolysis of sphingomyelin has been found to generate important signals regulating cell proliferation, differentiation and apoptosis. However, the enzymes responsible for digestion of dietary sphingomyelin have not been well documented. This study demonstrates the occurrence of a sphingomyelinase (SMase) in both human hepatic bile and gallbladder bile. The enzyme was equally found in both bacteria negative and positive bile samples and in samples obtained from patients with or without gallbladder diseases. A bacteria-free gallbladder bile was used for characterization. It was found that bile SMase hydrolyzed sphingomyelin to phosphorylcholine and ceramide with negligible activity against either phosphatidylcholine or p-nitrophenyl phosphate. The enzyme preferred an alkaline condition and the optimal pH was 9. The activity of this alkaline SMase was bile salt dependent and was fully activated by 4-6 mM bile salts. Triton X-100, the non-ionic detergent did not activate bile SMase. Ca2+ and Mg2+ ions had no significant effect at optimal bile salt concentration. The molecular mass of this enzyme was about 85 kDa as measured by Sephadex G200 gel chromatography. In conclusion, we demonstrated a SMase in bile which differs marked...Continue Reading

References

Jan 1, 1975·Methods in Enzymology·W Stoffel
Jan 12, 1976·Biochemical and Biophysical Research Communications·S Gatt
Jan 1, 1991·Progress in Lipid Research·R N Kolesnick
Dec 1, 1990·Gastroenterology·N R Pattinson, K E Willis
Jan 1, 1989·Journal of Inherited Metabolic Disease·D A Gibbs
Nov 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T NakanoF Nakayama
Dec 18, 1968·Biochimica Et Biophysica Acta·A Nilsson
Oct 23, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Poulos, K Beckman
Mar 1, 1980·The Biochemical Journal·S Yedgar, S Gatt
Apr 1, 1994·Current Biology : CB·R H Michell, M J Wakelam
Jul 1, 1994·Scandinavian Journal of Gastroenterology·T Juvonen
Mar 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M L Booker, W W LaMorte
May 1, 1994·The Journal of Nutrition·D L DillehayA H Merrill
May 1, 1994·The Journal of Nutrition·E M SchmelzA H Merrill
Nov 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·B Rosemary Grant, Peter R Grant

❮ Previous
Next ❯

Citations

Sep 23, 2000·Chemistry and Physics of Lipids·T LevadeR Salvayre
Jun 23, 2001·Molecular and Biochemical Parasitology·S A LauerK Haldar
Oct 20, 2009·Lipids in Health and Disease·Lena OhlssonAke Nilsson
Aug 19, 2009·Chembiochem : a European Journal of Chemical Biology·Anke Gundula RothChristoph Arenz
Mar 16, 2011·Journal of Digestive Diseases·Rui-Dong Duan
Sep 12, 2009·Angewandte Chemie·Anke G RothChristoph Arenz
Oct 20, 2006·Biochimica Et Biophysica Acta·Antonio DelgadoGemma Fabrias
Nov 26, 2008·Progress in Lipid Research·Rui-Dong Duan, Ake Nilsson
Apr 3, 2003·Journal of Lipid Research·Rui-Dong DuanAke Nilsson
Mar 16, 2017·The Journal of Biological Chemistry·Alexei GorelikBhushan Nagar
May 12, 2000·Critical Care Medicine·X LinR Kolesnick
Jul 20, 2002·Inflammatory Bowel Diseases·Urban SjöqvistRobert Löfberg
Jun 19, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jun WuRui-Dong Duan
Jan 26, 2007·ChemMedChem·Antonio DelgadoGemma Fabriás
Jan 15, 2010·The American Journal of Clinical Nutrition·Lena OhlssonAke Nilsson
Nov 20, 1998·Journal of Pediatric Gastroenterology and Nutrition·L NybergO Hernell
Jul 8, 1999·The Journal of Nutrition·H VesperA H Merrill
Jun 11, 1998·The Journal of Biological Chemistry·X LinR Kolesnick
Jun 27, 1997·The Journal of Biological Chemistry·B Liu, Y A Hannun
Sep 23, 2000·Chemistry and Physics of Lipids·A Nilsson, R D Duan
Jan 12, 1999·Cellular Signalling·T OkazakiM Tashima
Mar 31, 1998·Biochimica Et Biophysica Acta·A Gómez-Muñoz
Apr 27, 1999·Biochimica Et Biophysica Acta·T Levade, J P Jaffrézou
Jun 1, 2000·Biochimica Et Biophysica Acta·A HuwilerK Sandhoff
Jan 18, 2003·Biochimica Et Biophysica Acta·Nathalie Andrieu-Abadie, Thierry Levade
Dec 16, 2006·Archives of Biochemistry and Biophysics·Sergio F MartínYusuf A Hannun
Jan 6, 2007·Biochimica Et Biophysica Acta·R D DuanA Nilsson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis