PMID: 1909322Sep 1, 1991

Identification of an alternative nitrogenase system in Rhodospirillum rubrum

Journal of Bacteriology
L J Lehman, G P Roberts

Abstract

A second nitrogenase activity has been demonstrated in Rhodospirillum rubrum. This nitrogenase is expressed whenever a strain lacks an active Mo nitrogenase because of physiological or genetic inactivation. The alternative nitrogenase is able to support growth on N2 in the absence of fixed N. V does not stimulate, nor does Mo or W inhibit, growth or activity under the conditions tested. The proteins responsible for this activity were identified by electrophoretic and immunological properties. The synthesis of these proteins was repressed by NH4+. The alternative nitrogenase reductase is ADP ribosylated in response to darkness by the system that regulates the activity of the Mo nitrogenase. The genes for the alternative nitrogenase have been cloned, and the alternative nitrogenase reductase has been expressed in an in vitro transcription-translation system.

References

Sep 3, 2011·Standards in Genomic Sciences·A Christine MunkDavid C Schwartz
Jan 5, 1996·The Journal of Biological Chemistry·Y HeG P Roberts
Oct 17, 2018·Molecular Microbiology·Lisa DemtröderBernd Masepohl
Feb 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael J Gray, Jorge C Escalante-Semerena
Jan 18, 2018·Nature Microbiology·Yanning ZhengCaroline S Harwood
Jan 1, 1994·Critical Reviews in Biotechnology·R M AllenV K Shah
Apr 10, 2018·Metallomics : Integrated Biometal Science·Florence MusJohn W Peters
Jul 15, 2020·Annual Review of Microbiology·Caroline S Harwood
Apr 2, 2008·Applied and Environmental Microbiology·Doris A BetancourtPaul E Bishop

Related Concepts

Gene Expression Regulation, Bacterial
Post-Translational Protein Processing
Oxidase
Nitrogenase Activity
CP2 nitrogenase
Genes, Bacterial
Nitrogenase reductase
2D Polyacrylamide Gel Electrophoresis
Diazotrophy
Restriction Mapping

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