Identification of an amino acid region supporting specific methionyl-tRNA synthetase: tRNA recognition.

Journal of Molecular Biology
P MellotG Fayat

Abstract

Site-directed nuclease digestion and nonsense mutations of the Escherichia coli metG gene were used to produce a series of C-terminal truncated methionyl-tRNA synthetases. Genetic complementation studies and characterization of the truncated enzymes establish that the methionyl-tRNA synthetase polypeptide (676 residues) can be reduced to 547 residues without significant effect on either the activity or the stability of the enzyme. The truncated enzyme (M547) appears to be similar to a previously described fully active monomeric from of 64,000 Mr derived from the native homodimeric methionyl-tRNA synthetase (2 x 76,000 Mr) by limited trypsinolysis in vitro. According to the crystallographic three-dimensional structure at 2.5 A resolution of this trypsin-modified enzyme, the polypeptide backbone folds into two domains. The former, the N-domain, contain a crevice that is believed to bind ATP. The latter, the C-domain, has a 28 C-residue extension (520 to 547), which folds back, toward the N-domain and forms an arm linking the two domains. This study shows that upon progressive shortening of this C-terminal extension, the enzyme thermostability decreases. This observation, combined with the study of several point mutations, allows ...Continue Reading

References

Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P CarterG Winter
Sep 1, 1986·Biochimie·C HountondjiS Blanquet
Jun 13, 1986·Journal of Chromatography·J M SchmitterM Anselme
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Jan 1, 1984·Molecular & General Genetics : MGG·E T Palva, T J Silhavy
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P WalterF Fasiolo
Dec 25, 1983·Journal of Molecular Biology·D M BlowC Zelwer
Aug 1, 1982·Biochimie·O HuismanS Casaregola
Feb 15, 1982·Journal of Molecular Biology·C ZelwerS Brunie

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Citations

Feb 15, 2013·Journal of the American Chemical Society·Alborz MahdaviDavid A Tirrell
Sep 13, 2005·Nature Structural & Molecular Biology·Kotaro NakanishiOsamu Nureki
Apr 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Thomas E JonesTao Pan
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hany S GirgisSaeed Tavazoie
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A BrevetS Blanquet
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T MeinnelG Fayat
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y M HouP Schimmel
Nov 30, 2012·Nucleic Acids Research·Marie NaveauEmmanuelle Schmitt
Jun 1, 1997·European Journal of Biochemistry·E SchmittS Blanquet
Jul 21, 2004·Journal of Bacteriology·Yisheng KangFrederick R Blattner
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·Kristi L KiickCarolyn R Bertozzi
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·R W AlexanderP Schimmel
Apr 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Tania BourMagali Frugier
Sep 8, 2016·Scientific Reports·Alejandro Manzano-Marín, Amparo Latorre
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·D D Buechter, P Schimmel
Jun 26, 1999·The Journal of Biological Chemistry·J SoutourinaS Blanquet
Mar 29, 2001·The Journal of Biological Chemistry·N TakeuchiS Blanquet
Jan 10, 2020·PLoS Computational Biology·Vaitea OpuuThomas Simonson
Feb 6, 2004·The Journal of Biological Chemistry·Laure YatimeYves Mechulam

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