Identification of an amino acid residue on influenza C virus M1 protein responsible for formation of the cord-like structures of the virus

The Journal of General Virology
Y MurakiS Hongo

Abstract

Influenza C virus-like particles (VLPs) have been generated from cloned cDNAs. A cDNA of the green fluorescent protein (GFP) gene in antisense orientation was flanked by the 5' and 3' non-coding regions of RNA segment 5 of the influenza C virus. The cDNA cassette was inserted between an RNA polymerase I promoter and terminator of the Pol I vector. This plasmid DNA was transfected into 293T cells together with plasmids encoding virus proteins of C/Ann Arbor/1/50 or C/Yamagata/1/88. Transfer of the supernatants of the transfected 293T cells to HMV-II cells resulted in GFP expression in the HMV-II cells. The quantification of the GFP-positive HMV-II cells indicated the presence of approximately 10(6) VLPs (ml supernatant)(-1). Cords 50-300 microm in length were observed on transfected 293T cells, although the cords were not observed when the plasmid for M1 protein of C/Ann Arbor/1/50 was replaced with that of C/Taylor/1233/47. A series of transfection experiments with plasmids encoding M1 mutants of C/Ann Arbor/1/50 or C/Taylor/1233/47 showed that an amino acid at residue 24 of the M1 protein is responsible for cord formation. This finding provides direct evidence for a previous hypothesis that M1 protein is involved in the format...Continue Reading

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Citations

Nov 26, 2010·Journal of Virology·Takatoshi FurukawaSeiji Hongo
Dec 17, 2009·Journal of Virology·Yasushi MurakiSeiji Hongo
Sep 28, 2012·Journal of Virology·Emi TakashitaYoko Matsuzaki
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Sep 10, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Yasushi MurakiYoshiro Ohara
Mar 1, 2011·Virus Research·Yasushi MurakiYoshiro Ohara
Jul 2, 2016·The Journal of General Virology·Bernadeta DadonaiteEdward C Hutchinson
Jan 13, 2018·Journal of Virology·Sumiho NakatsuYoshihiro Kawaoka
Jul 8, 2016·Journal of Virology·Yoko MatsuzakiHidekazu Nishimura
Sep 8, 2017·Journal of Virology·Takanari GotoSeiji Hongo
Jun 14, 2008·Journal of Medical Virology·S GouarinF Freymuth
Jul 22, 2019·Bioscience Reports·Ismail DahmaniSalvatore Chiantia
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Feb 28, 2020·Journal of Virology·Hiroho IshidaTaisuke Horimoto
Jul 3, 2019·Cells·Janet To, Jaume Torres
Mar 18, 2021·Nature Communications·Steinar HalldorssonPeter B Rosenthal
Aug 19, 2021·Journal of Virology·Ko SatoHidekazu Nishimura

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