PMID: 562799Aug 1, 1977Paper

Identification of an androgen receptor in the cytosol of the female Mastomys prostate

Molecular and Cellular Endocrinology
R GhanadianG D Chisholm

Abstract

An in vivo and in vitro study was carried out on the prostate from the female Praomys (Mastomys) natalensis to identify and characterize the binding of androgens within the cytoplasm. The labelled cytosol was prepared and subjected to gel exclusion chromatography and density gradient centrifugation. A macromolecular protein associated with the radioactivity was isolated on Sephadex G-200. Subsequent analysis of the steroid receptor complex showed that the major part of the radioactive steroid (64 percent) was dihydrotestosterone. This binding was inhibited by unlabelled testosterone and could not be demonstrated in liver cytosol. Characterization of this dihydrotestosterone receptor complex revealed a sedimentation coefficient of 4.6 s in the presence of a high salt solution (0.4 M KCl). The complex aggregated in the absence of 0.4 M KCl and sedimented preferentially from 5.6-7.4 s together with polydisperse aggregates of higher sedimentation coefficients. The use of this animal as an experimental model for hormonal studies on the prostate is suggested.

References

Dec 1, 1969·The Journal of Endocrinology·W I Mainwaring
Feb 20, 1970·Biochemical and Biophysical Research Communications·E E Baulieu, I Jung

Citations

Jun 1, 1981·Journal of Steroid Biochemistry·J Pelletier, A Caraty
Nov 1, 1979·Molecular and Cellular Endocrinology·C Monet-KuntzA Locatelli
Jan 1, 1977·British Journal of Urology·R GhanadianG D Chisholm

Related Concepts

Castration
Cytoplasmic Matrix
Liver
Prostate
Androgen Receptor
Receptors, Steroid
Family Dipodidae
Dihydroepitestosterone
Sterotate

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