Identification of an anti-SARS-CoV-2 receptor-binding domain-directed human monoclonal antibody from a naïve semisynthetic library.

The Journal of Biological Chemistry
Hilal Ahmad ParrayRajesh Kumar

Abstract

There is a desperate need for safe and effective vaccines, therapies, and diagnostics for SARS- coronavirus 2 (CoV-2), the development of which will be aided by the discovery of potent and selective antibodies against relevant viral epitopes. Human phage display technology has revolutionized the process of identifying and optimizing antibodies, providing facile entry points for further applications. Herein, we use this technology to search for antibodies targeting the receptor-binding domain (RBD) of CoV-2. Specifically, we screened a naïve human semisynthetic phage library against RBD, leading to the identification of a high-affinity single-chain fragment variable region (scFv). The scFv was further engineered into two other antibody formats (scFv-Fc and IgG1). All three antibody formats showed high binding specificity to CoV-2 RBD and the spike antigens in different assay systems. Flow cytometry analysis demonstrated specific binding of the IgG1 format to cells expressing membrane-bound CoV-2 spike protein. Docking studies revealed that the scFv recognizes an epitope that partially overlaps with angiotensin-converting enzyme 2 (ACE2)-interacting sites on the CoV-2 RBD. Given its high specificity and affinity, we anticipate th...Continue Reading

References

Nov 17, 2012·BMC Biotechnology·Rajesh KumarKalpana Luthra
Jun 13, 2014·Clinical and Vaccine Immunology : CVI·Jean-Louis ExclerStanley A Plotkin
Dec 3, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Thierry Burnouf, Jerard Seghatchian
Jun 23, 2015·Methods in Enzymology·Anne SpriestersbachBarbara Maertens
Nov 27, 2015·Journal of Immunological Methods·Rabab M Abou El-MagdDavid S Wishart
Dec 18, 2015·Blood Transfusion = Trasfusione Del Sangue·Giuseppe MaranoGiuliano Grazzini
Oct 4, 2016·Drug Design, Development and Therapy·Abhinav KanwalSanjay K Banerjee
Mar 30, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Salman BagheriZahra Sharifzadeh
Jan 19, 2018·Annual Review of Immunology·Annie Elong Ngono, Sujan Shresta
Nov 16, 2018·Biologicals : Journal of the International Association of Biological Standardization·Surachet BenjathummarakPannamthip Pitaksajjakul
Jun 7, 2019·International Journal of Biological Macromolecules·Rajesh KumarKalpana Luthra
Dec 13, 2019·Journal of Virology·Yushun WanFang Li
Feb 23, 2020·Applied Microbiology and Biotechnology·Rajesh KumarHilal Ahmad Parray
Mar 14, 2020·The Journal of Clinical Investigation·Arturo Casadevall, Liise-Anne Pirofski
Apr 30, 2020·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Bethany L Brown, Jeffrey McCullough
May 1, 2020·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Susanna FelsensteinChristian M Hedrich
May 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jian ShangFang Li

❮ Previous
Next ❯

Citations

Mar 6, 2021·Frontiers in Genetics·Sreyashi MajumdarSudipto Saha
May 11, 2021·Frontiers in Molecular Biosciences·Long Min, Qiu Sun
Jun 24, 2021·Journal of Cellular Biochemistry·Abdolreza EsmaeilzadehMajid Ahmadi
Jul 3, 2021·Viruses·Esen SokulluBenoit Coulombe
Jul 27, 2021·Frontiers in Cellular and Infection Microbiology·Kristian Daniel Ralph RothMichael Hust
Dec 11, 2020·Journal of Proteome Research·Suvobrata Chakravarty
Aug 24, 2021·Applied Microbiology and Biotechnology·Hilal Ahmad ParrayRajesh Kumar
Sep 10, 2021·Cellular & Molecular Immunology·Lanying DuXiujuan Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
phage display
ELISA
biosensor
flow cytometry
immunoprecipitation
co-IP
biolayer interferometry
glycosylation
transfection

Software Mentioned

ForteBio
BLAST

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

BioRxiv : the Preprint Server for Biology
Emanuel K Peter, Alexander Schug
Antimicrobial Agents and Chemotherapy
Keivan ZandiRaymond F Schinazi
The Journal of Clinical Investigation
William R MorgenlanderH Benjamin Larman
Pharmacological Reports : PR
Alberto Boretti
© 2021 Meta ULC. All rights reserved