PMID: 2493152Feb 1, 1989Paper

Identification of an assembly domain in the small subunit of ribulose-1,5-bisphosphate carboxylase

Proceedings of the National Academy of Sciences of the United States of America
C C WasmannJ A Ostrem

Abstract

The mature small subunit (SSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) in higher plants contains a highly conserved sequence of 16 amino acids that is absent in the SSUs of cyanobacteria. To determine whether this region of the SSU of higher plants has a specific function, portions of the SSU genes (rbcS) of pea (Pisum sativum) and the cyanobacterium Anacystis nidulans were fused to create chimeric genes that either lacked or contained the coding sequence for the 16 conserved amino acids. Precursor proteins synthesized in vitro from the chimeric genes were incubated with isolated pea chloroplasts to assay import and assembly into the holoenzyme. Fusion proteins lacking the 16-amino acid sequence were imported and processed but failed to assemble with endogenous large subunit. Addition of the region from a pea rbcS containing the 16 amino acids to the rbcS of Anacystis enabled the imported SSU fusion protein to assemble with pea large subunit. This 16-amino acid sequence is encoded by a separate exon in certain rbcS genes of higher plants. We propose that the conserved 16-amino acid sequence constitutes a domain acquired to facilitate assembly of the eukaryotic holoenzyme.

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Citations

Nov 1, 1990·Photosynthesis Research·S M Newman, R A Cattolico
Apr 2, 2013·Photosynthesis Research·Amanda P Cavanagh, David S Kubien
Jun 5, 2003·Archives of Biochemistry and Biophysics·Robert J Spreitzer
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J H FitchenL McIntosh
Sep 8, 2009·The Journal of Biological Chemistry·Todor Genkov, Robert J Spreitzer
Apr 12, 2012·Molecular Biology and Evolution·Lei GongJonathan F Wendel
Sep 12, 2002·Annual Review of Plant Biology·Robert J Spreitzer, Michael E Salvucci
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y C DuR J Spreitzer
Jul 13, 2011·Journal of Molecular Biology·Michiel van LunInger Andersson

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