Identification of an attenuated barley stripe mosaic virus for the virus-induced gene silencing of pathogenesis-related wheat genes

Plant Methods
Leann M BuhrowMichele C Loewen

Abstract

Virus-induced gene silencing (VIGS) has become an emerging technology for the rapid, efficient functional genomic screening of monocot and dicot species. The barley stripe mosaic virus (BSMV) has been described as an effective VIGS vehicle for the evaluation of genes involved in wheat and barley phytopathogenesis; however, these studies have been obscured by BSMV-induced phenotypes and defense responses. The utility of BSMV VIGS may be improved using a BSMV genetic background which is more tolerable to the host plant especially upon secondary infection of highly aggressive, necrotrophic pathogens such as Fusarium graminearum. BSMV-induced VIGS in Triticum aestivum (bread wheat) cv. 'Fielder' was assessed for the study of wheat genes putatively related to Fusarium Head Blight (FHB), the necrotrophism of wheat and other cereals by F. graminearum. Due to the lack of 'Fielder' spike viability and increased accumulation of Fusarium-derived deoxynivalenol contamination upon co-infection of BSMV and FHB, an attenuated BSMV construct was generated by the addition of a glycine-rich, C-terminal peptide to the BSMV γ b protein. This attenuated BSMV effectively silenced target wheat genes while limiting disease severity, deoxynivalenol con...Continue Reading

References

Jul 1, 1995·Molecular Plant-microbe Interactions : MPMI·R H ProctorS P McCormick
May 1, 1996·The Journal of General Virology·R G Donald, A O Jackson
Jun 23, 1998·The Plant Cell·M T RuizD C Baulcombe
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Steve HolzbergGregory P Pogue
Apr 3, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·X ShenH Ohm
Apr 26, 2003·Trends in Biochemical Sciences·Ahmet M Denli, Gregory J Hannon
Feb 21, 2004·The EMBO Journal·Lóránt LakatosJózsef Burgyán
Jul 31, 2004·Annual Review of Phytopathology·W E Durrant, X Dong
Aug 19, 2004·The Plant Journal : for Cell and Molecular Biology·Tessa M Burch-SmithS P Dinesh-Kumar
Apr 30, 2009·Annual Review of Phytopathology·Andrew O JacksonMi Yeon Lee
Jun 5, 2010·Molecular Plant-microbe Interactions : MPMI·Ragiba MakandarJyoti Shah
Jun 15, 2011·Annual Review of Phytopathology·Alexandre Robert-SeilaniantzJonathan D G Jones
Nov 25, 2011·Molecular Plant-microbe Interactions : MPMI·Ragiba MakandarJyoti Shah
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen
Jan 3, 2013·Molecular Plant Pathology·Barbara SchermQuirico Migheli
Jun 8, 2013·Frontiers in Plant Science·Nicolas DenancéAntonio Molina
Aug 8, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Maria BuerstmayrHermann Buerstmayr
Dec 10, 2013·TheScientificWorldJournal·Arash NezhadahmadiGolam Faruq
Jan 9, 2014·Frontiers in Plant Science·Sergi Munné-Bosch, Maren Müller
Jan 22, 2015·BMC Plant Biology·Sylvia UlfertsUlrich Schaffrath
May 15, 2015·Plant Molecular Biology·Chae Woo Lim, Sung Chul Lee

❮ Previous
Next ❯